Cytotoxicity of 5 endodontic sealers on L929 cell line and human dental pulp cells

• Published in: International endodontic journal 2011-07, Vol.44 (7), p.626-634
• Main Authors: Karapınar-Kazandağ, M., Bayrak, Ö. F., Yalvaç, M. E., Ersev, H., Tanalp, J., Şahin, F., Bayırlı, G.
• Format: Article
• Language: English
• Subjects: Acrylic Resins - chemistry; Activ GP; Adult; Animals; biocompatibility; Cell Culture Techniques; Cell Line; Cell Proliferation - drug effects; Cell Survival - drug effects; Cells, Cultured; Colorimetry; Composite Resins - chemistry; cytotoxicity; Dental Cements - chemistry; Dental Cements - toxicity; Dental Pulp - cytology; Dental Pulp - drug effects; EndoREZ; Epiphany; Epoxy Resins - chemistry; Fibroblasts - drug effects; human dental pulp cell; Humans; Humidity; Immunohistochemistry; Marine; Materials Testing; Mice; Mice, Inbred C3H; Resin Cements - chemistry; Root Canal Filling Materials - chemistry; Root Canal Filling Materials - toxicity; root canal sealer; Spectrophotometry; Temperature; Time Factors
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2011.01863.x

Karapınar‐Kazandağ M, Bayrak ÖF, Yalvaç ME, Ersev H, Tanalp J, Şahin F, Bayırlı G. Cytotoxicity of 5 endodontic sealers on L929 cell line and human dental pulp cells. International Endodontic Journal, 44, 626–634, 2011. Aim  To investigate the cytotoxicity of five root canal sealers on L929 mouse fibroblasts and primary human dental pulp cells. Methodology  Cylindrical specimens of AH Plus (Dentsply De Trey GmbH, Konstanz, Germany), RoekoSeal (Coltène Whaledent, Langenau, Germany), EndoREZ (Ultradent Products Inc., South Jordan, UT, USA), Epiphany (Pentron Clinical Technologies, LLCC, Wallingford, CT, USA) and Activ GP (Brasseller Inc., USA, Savannah, GA, USA) were kept at 37 °C in a humidified atmosphere of 5% CO2 for thrice the length of the setting time given by the manufacturer. Extraction of specimens was performed after setting in cell growth medium for 1, 4 and 7 days. Undiluted, 50% and 25% diluted eluates were incubated with cultured cells for 24 and 72 h. Cytotoxicity was assessed using MTS colorimetric bioassay. Kruskal–Wallis test and post hoc Dunn’s multiple comparison test were used to compare the sealers and diluted/undiluted eluates in terms of cell viability (% of control). Friedman test and post hoc Dunn’s multiple comparison test were performed to compare extraction periods. Wilcoxon test was utilized in comparing 24‐ and 72‐h readings. Results  Undiluted 1‐day eluate of Activ GP was significantly more cytotoxic than all other sealers (P