Polymorphisms of the T-cell immunoglobulin and mucin domain molecule-3 are not associated with autoimmune Graves' disease in a Chinese Han Population

• Published in: Clinical biochemistry 2012-12, Vol.45 (18), p.1624-1628
• Main Authors: Liang, Tao, Wang, Xiao Bei, Cai, Peng Cheng, Tang, Zhao Ming, Chen, Zhi Zhong, Hu, Li Hua
• Format: Article
• Language: English
• Subjects: Adult; Asian Continental Ancestry Group - genetics; Autoimmune diseases; Biological and medical sciences; Case-Control Studies; China; Demography; Endocrinopathies; Ethnic Groups - genetics; Female; Gene expression; Gene frequency; Gene polymorphism; Genetic Association Studies; Genetic Predisposition to Disease; genomics; Graves Disease - genetics; Graves' disease; Hepatitis A Virus Cellular Receptor 2; Humans; Immunoglobulins; Investigative techniques, diagnostic techniques (general aspects); Leukocytes, Mononuclear - metabolism; Lymphocytes T; Male; Medical sciences; Membrane Proteins - genetics; mucin; Non tumoral diseases. Target tissue resistance. Benign neoplasms; Peripheral blood; Polymerase Chain Reaction; Polymorphism, Single Nucleotide - genetics; Polymorphisms; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Single-nucleotide polymorphism; Thyroid. Thyroid axis (diseases); TIM-3
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2012.08.004

This study aimed to investigate the association between polymorphisms of T-cell immunoglobulin and mucin domain molecule-3 (TIM-3) and Graves' disease (GD) in a Chinese population. Genomic DNA was extracted from peripheral blood cells of the 182 GD patients and 150 control subjects. The TIM-3 gene polymorphic sites were genotyped. We also analyzed the relationships between the genotypes of each SNP and serum specific clinical variables. To detect whether the variants were associated with the TIM-3 expression, we further studied 40 patients by using the method of real-time quantitative reverse-transcription polymerase chain reaction (real-time RT-PCR). The genotype and allele frequency of each polymorphic site were not significantly different between GD and control individuals. Furthermore, it also showed no relationship between the variants and TIM-3 mRNA expression. Our results demonstrated that the polymorphisms of TIM-3 gene may not contribute to GD susceptibility in the Chinese Han population.