A simple approach for enhanced immune response using engineered dendritic cell targeted nanoparticles

Highlights ► A simple strategy to formulate DC targeted PLGA nanoparticles (NPs) is reported. ► Ovalbumin-loaded biotinylated NPs were formulated using biotin–PEG–PLGA polymer. ► A recombinant bifunctional fusion protein (bfFp) is used as a DC targeting ligand. ► BfFp with OVA loaded NPs yields a ve...

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Bibliographic Details
Published in:Vaccine 2012-11, Vol.30 (50), p.7292-7299
Main Authors: Raghuwanshi, Dharmendra, Mishra, Vivek, Suresh, Mavanur R, Kaur, Kamaljit
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antigens
Antigens - immunology
Antigens - metabolism
Bifunctional fusion protein
Biotin
Cytokines - secretion
DEC-205 receptor
Dendritic cells
Dendritic Cells - immunology
Dendritic Cells - metabolism
Drug Carriers - metabolism
Fusion protein
Helper cells
Immune response
Immune system
Immunization
Immunoglobulin G
Immunoglobulin G - blood
interferon-gamma
Interleukin 2
Leukocytes, Mononuclear - immunology
Lymphocytes T
Mice
Monoclonal antibodies
Nanoparticles
Ovalbumin
Ovalbumin - immunology
Ovalbumin - metabolism
PLGA nanoparticles
polylactide-co-glycolide
Polymers
Protein Binding
Proteins
Recombinant Proteins - metabolism
Spleen - immunology
Splenocytes
Studies
systems engineering
Vaccine delivery
Vaccines
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Summary:Highlights ► A simple strategy to formulate DC targeted PLGA nanoparticles (NPs) is reported. ► Ovalbumin-loaded biotinylated NPs were formulated using biotin–PEG–PLGA polymer. ► A recombinant bifunctional fusion protein (bfFp) is used as a DC targeting ligand. ► BfFp with OVA loaded NPs yields a versatile two-component antigen delivery system. ► DC targeted formulations along with αCD40 significantly enhance immune responses.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2012.09.036