Proteomic evaluation of potentiated sulfa treatment on gilthead sea bream (Sparus aurata L.) liver

• Published in: Aquaculture 2013-02, Vol.376-379, p.36-44
• Main Authors: Varó, Inmaculada, Navarro, Juan C., Rigos, George, Del Ramo, José, Calduch-Giner, Josep Alvar, Hernández, Adoración, Pertusa, José, Torreblanca, Amparo
• Format: Article
• Language: English
• Subjects: 2D-DIGE; Agnatha. Pisces; aldehyde dehydrogenase; Animal and plant ecology; Animal aquaculture; Animal productions; Animal, plant and microbial ecology; antioxidant activity; antioxidants; apolipoprotein A-I; Aquaculture; Biological and medical sciences; biomarkers; bream; carbohydrate metabolism; combination drug therapy; Cytotoxicity; Drugs; electrophoresis; farmed fish; fatty acid-binding proteins; Fish; fish culture; Fundamental and applied biological sciences. Psychology; gene expression; General aspects; Gilthead sea bream; hepatotoxicity; juveniles; lipid metabolism; Liver; Marine; marine fish; Mass spectrometry (MS); oral administration; oxidation; Pathogens; Potentiated sulfa; protein synthesis; Proteins; Proteomic; Proteomics; Sea water ecosystems; Sparus aurata; stress response; sulfonamides; Synecology; temperature; transaminases; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2012.11.012

Potentiated sulfa drugs are a combination of sulfonamide and pyrimidine potentiators. They are currently used against fish bacterial pathogens in Mediterranean marine fish farming. The present work aimed studying the potential hepatotoxicity of a combination of sulfadiazine (SDZ) and trimethoprim (TMP) in gilthead sea bream juveniles after oral administration, at the recommended ratio of 5:1 (SDZ/TMP), equivalent to a dose of 30mgkg−1fishday−1, for 10days at 19°C temperature. Electrophoresis (DIGE) technology coupled with MS was used to identify possible markers of hepatotoxicity of this treatment. The results obtained show significant changes in the expression of 41 proteins by treatment (p≤0.02). Among these proteins, 14 increased in abundance, and 27 decreased with respect to the control group. Proteins showing differential expression with respect to the control were identified by PMF and/or LC-MS/MS and database research. Proteins like apolipoprotein A-I and fatty acid binding protein (lipid metabolism and transport, and antioxidant role), phosphoglucomutase 1 (carbohydrate metabolism), elongation factor 1-alpha (protein biosynthesis and antioxidant role), mitochondrial aldehyde dehydrogenase (oxidation regulation activity and antioxidant role) and ypbc-32-D06 (aminotransferases), were differentially expressed in treated fish. These proteins have not been associated before with potentiated sulfa effect in farmed fish. However, they are frequently found differentially expressed as a characteristic cellular/tissue stress response under different experimental conditions, making difficult their use as specific biomarkers for this treatment. ► Molecular study about effects of potentiated sulfa on liver of gilthead sea bream. ► A set of 41 proteins was differentially expressed in response to treatment. ► The proteins identified have not been related before to potentiated sulfa effect. ► The proteins identified are involved in common cell/tissue stress response.