Freeze-drying as a preserving preparation technique for in vitro testing of human skin

In vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In thi...

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Bibliographic Details
Published in:Experimental dermatology 2013-01, Vol.22 (1), p.54-56
Main Authors: Franzen, Lutz, Vidlářová, Lucie, Kostka, Karl-Heinz, Schaefer, Ulrich F., Windbergs, Maike
Format: Article
Language:English
Subjects:
Area Under Curve
Caffeine - chemistry
Caffeine - pharmacokinetics
Calorimetry, Differential Scanning
Confidence Intervals
confocal Raman microscopy
Epidermis - metabolism
Freeze Drying
human skin
Humans
in vitro in vivo comparison
infrared spectroscopy
Microscopy, Confocal
Microscopy, Polarization
Skin - metabolism
Spectrophotometry, Infrared
Spectrum Analysis, Raman
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Summary:In vitro testing of drugs with excised human skin is a valuable prerequisite for clinical studies. However, the analysis of excised human skin presents several obstacles. Ongoing drug diffusion, microbial growth and changes in hydration state influence the results of drug penetration studies. In this work, we evaluate freeze‐drying as a preserving preparation method for skin samples to overcome these obstacles. We analyse excised human skin before and after freeze‐drying and compare these results with human skin in vivo. Based on comprehensive thermal and spectroscopic analysis, we demonstrate comparability to in vivo conditions and exclude significant changes within the skin samples due to freeze‐drying. Furthermore, we show that freeze‐drying after skin incubation with drugs prevents growth of drug crystals on the skin surface due to drying effects. In conclusion, we introduce freeze‐drying as a preserving preparation technique for in vitro testing of human skin.
ISSN:0906-6705
1600-0625
DOI:10.1111/exd.12058