Efficacy of disinfecting solutions in removing biofilms from polyvinyl chloride tracheostomy tubes

Objectives/Hypothesis: Bacterial biofilms are prevalent in pediatric tracheostomy tubes (TTs) and are not completely cleared by standard cleaning with gauze and household detergents. We aimed to examine the effectiveness of different disinfecting solutions to remove Staphylococcus aureus (SA) and Ps...

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Bibliographic Details
Published in:The Laryngoscope 2013-01, Vol.123 (1), p.259-263
Main Authors: Silva, Rodrigo C., Carver, Ryan A., Ojano-Dirain, Carolyn P., Antonelli, Patrick J.
Format: Article
Language:English
Subjects:
Bacterial biofilms
Biofilms
Biofilms - drug effects
Colony Count, Microbial
Disinfectants - pharmacology
disinfection
Equipment Contamination
Humans
Microscopy, Electron, Scanning
Ostomy
Pediatrics
Polyvinyl Chloride
Prospective Studies
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - growth & development
Staphylococcus aureus - drug effects
Staphylococcus aureus - growth & development
Tracheostomy - instrumentation
tracheostomy tube
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Summary:Objectives/Hypothesis: Bacterial biofilms are prevalent in pediatric tracheostomy tubes (TTs) and are not completely cleared by standard cleaning with gauze and household detergents. We aimed to examine the effectiveness of different disinfecting solutions to remove Staphylococcus aureus (SA) and Pseudomonas aerginosa (PA) biofilms from TTs. Study Design: Prospective, controlled, in vitro microbiologic study. Methods: Uniform coupons obtained from polyvinyl chloride (PVC) pediatric TTs were briefly exposed to human plasma. The samples were incubated in growth media with either PA or SA for 7 days, and total bacterial growth was monitored by media turbidity. Five sets of 18 coupons each were exposed for 5 minutes to one of five different solutions: 2% aqueous chlorhexidine gluconate solution, 0.3% aqueous sodium hypochlorite, Polident denture cleanser, 3% hydrogen peroxide, or preservative‐free phosphate‐buffered saline (PBS) as a negative control. Biofilm presence was measured with bacterial counts, and surface integrity was assessed with scanning electron microscopy (SEM). Results: All treatments significantly reduced mean SA counts (P =
ISSN:0023-852X
1531-4995
DOI:10.1002/lary.23612