Use of Fluorescent Stainings and Flow Cytometry for Canine Semen Assessment

Contents Over the last few decades, appreciable progress has been noted in canine semen assessment techniques. The common use of accurate and sensitive diagnostic methods, such as computer‐assisted sperm analysis (CASA), flow cytometry and sperm penetration tests have become routine procedures in sp...

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Bibliographic Details
Published in:Reproduction in domestic animals 2012-12, Vol.47 (s6), p.215-221
Main Authors: Niżański, W, Partyka, A, Rijsselaere, T
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Chromatin
DNA
DNA Fragmentation
Dogs
Flow cytometry
Flow Cytometry - veterinary
Fluorescence
Fluorescent Dyes - chemistry
Lipid Peroxidation
Mitochondria
Semen - physiology
Semen Analysis - methods
Semen Analysis - veterinary
Staining and Labeling
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Summary:Contents Over the last few decades, appreciable progress has been noted in canine semen assessment techniques. The common use of accurate and sensitive diagnostic methods, such as computer‐assisted sperm analysis (CASA), flow cytometry and sperm penetration tests have become routine procedures in specialized andrology laboratories. Many fluorescent probes have been applied to the assessment of specific sperm characteristics in dogs. Flow cytometry enables the observation of cell characteristics such as size, shape and function of the spermatozoon, that can be revealed by a fluorochrome or fluorescent label. The analysis of events detected on dot plots gives accurate and highly reliable information on membrane integrity, acrosomal status, mitochondrial activity, capacitation status, lipid peroxidation, apoptosis and DNA damage. Despite the development of these modern and accurate tools, it is still questionable if the ideal method of semen evaluation, allowing predicting of the fertilizing potential of semen, has been established.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12048