The Neuroprotective Effect of Platelet‐rich Plasma on Erectile Function in Bilateral Cavernous Nerve Injury Rat Model

Neurogenic erectile dysfunction resulting from cavernous nerve (CN) injury is a major complication caused by radical prostatectomy. The use of platelet‐rich plasma (PRP) on the nerve‐injured site has shown promising results for the nerve regeneration. However, the effects of PRP injection in corpus...

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Bibliographic Details
Published in:Journal of sexual medicine 2012-11, Vol.9 (11), p.2838-2848
Main Authors: Wu, Chien‐Chih, Wu, Yi‐No, Ho, Hsiu‐O, Chen, Kuo‐Chiang, Sheu, Ming‐Thau, Chiang, Han‐Sun
Format: Article
Language:English
Subjects:
Animals
Apoptosis - genetics
Apoptosis - physiology
Caspase 3 - genetics
Cavernous Nerve
Disease Models, Animal
Electric Stimulation
Gene Expression - genetics
Growth Factor
Impotence, Vasculogenic - genetics
Impotence, Vasculogenic - physiopathology
Male
Muscle Denervation
Nerve Crush
Nerve Regeneration - genetics
Nerve Regeneration - physiology
Neurogenic Erectile Dysfunction
Neuroprotective
Penis - innervation
Platelet-rich Plasma
Platelet-Rich Plasma - physiology
Rats
Rats, Sprague-Dawley
RNA, Messenger - genetics
Transforming Growth Factor beta1 - genetics
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Summary:Neurogenic erectile dysfunction resulting from cavernous nerve (CN) injury is a major complication caused by radical prostatectomy. The use of platelet‐rich plasma (PRP) on the nerve‐injured site has shown promising results for the nerve regeneration. However, the effects of PRP injection in corpus cavernosum after bilateral CN injury have never been investigated. To assess the neuroprotective effect of PRP injection in corpus cavernosum after bilateral CN injury. Male Sprague‐Dawley rats were randomly divided into three groups: Group I underwent sham operation, while the remaining two groups underwent bilateral CN crush. Crush injury groups were treated at the time of injury with an application of PRP or normal saline only injection in the corpus cavernosum, respectively. Four weeks later, erectile function (EF) was assessed by CN electrosimulation, and CNs as well as penile tissue were collected for histology. Intracavernous pressure (ICP) monitored during electrical stimulation of CNs; myelinated axons number of CNs and dorsal penile nerve; collagen type change, number of apoptotic cells, and mRNA expression of caspase‐3 and transforming growth factor‐β1 (TGF‐β1) in the corpus cavernosum. Four weeks after surgery, in the vehicle‐only group, the functional evaluation showed a lower mean maximal ICP than that in the sham group (P 
ISSN:1743-6095
1743-6109
DOI:10.1111/j.1743-6109.2012.02881.x