Standardisation of metalloimmunoassay protocols for assessment of silver nanoparticle antibody conjugates

The application of silver nanoparticles to electrochemical metalloimmunoassays has previously been reported (Szymanski et al., 2009) and is now used in the electrochemical Argento immunoassay platform via UK Patent No. 2458420. The development of an immunoassay in this format requires the optimisati...

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Bibliographic Details
Published in:Journal of immunological methods 2013-01, Vol.387 (1-2), p.303-307
Main Authors: Wilson, P.K., Szymanski, M., Porter, R.
Format: Article
Language:English
Subjects:
Antibodies
Antibodies - chemistry
Antibodies - immunology
Antibodies, Immobilized - chemistry
Antibodies, Immobilized - immunology
Buffers
Cardiac markers
Electrochemical Techniques - methods
Electrochemical Techniques - standards
electrochemistry
Hydrogen-Ion Concentration
Immunoassay
Immunoassay - methods
Immunoassay - standards
Immunoassays
Metal Nanoparticles - chemistry
Metal Nanoparticles - standards
Metalloimmunoassay
nanoparticles
nanosilver
pH effects
Point-of-care diagnostics
Reference Standards
Reproducibility of Results
Silver
Silver - chemistry
Silver nanoparticles
Thiocyanates - chemistry
Time Factors
Troponin
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Summary:The application of silver nanoparticles to electrochemical metalloimmunoassays has previously been reported (Szymanski et al., 2009) and is now used in the electrochemical Argento immunoassay platform via UK Patent No. 2458420. The development of an immunoassay in this format requires the optimisation of the antibody to silver nanoparticle conjugation process. Issues such as pH, antibody concentration and other factors can affect the assay performance. In order to determine the effect of these variables it is necessary to understand and control the effects of other factors that may affect the assay signal. In this study a number of conditions which affect the assay signal were identified and methods developed to minimise variability in the assay signal, resulting in a standardised method allowing easy comparisons of silver nanoparticle conjugates prepared and assayed at different times.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2012.08.001