The influence of sex, gestational age, birth weight, blood transfusion, and timing of the heel prick on the pancreatitis-associated protein concentration in newborn screening for cystic fibrosis

• Published in: Journal of inherited metabolic disease 2013, Vol.36 (1), p.147-154
• Main Authors: Vernooij-van Langen, Annette M. M., Loeber, J. Gerard, Elvers, Bert, Triepels, Ralf H., Roefs, Jos, Gille, Johan J., Reijntjens, Sandra, Dompeling, Edward, Dankert-Roelse, Jeannette E.
• Format: Article
• Language: English
• Subjects: Age; Antigens, Neoplasm - metabolism; Biochemistry; Biological and medical sciences; Biomarkers - metabolism; Biomarkers, Tumor - metabolism; Birth Weight; Blood Transfusion; Blood transfusions; Cystic fibrosis; Cystic Fibrosis - blood; Cystic Fibrosis - diagnosis; Cystic Fibrosis - metabolism; Enzyme-linked immunosorbent assay; Errors of metabolism; Female; Gestational Age; Heel - blood supply; Human Genetics; Humans; Infant, Newborn; Infant, Premature - blood; Infant, Premature - metabolism; Infants; Internal Medicine; Lectins, C-Type - metabolism; Low-birth-weight; Male; Medical genetics; Medical sciences; Medical screening; Medicine; Medicine & Public Health; Metabolic Diseases; Miscellaneous hereditary metabolic disorders; Neonatal Screening - methods; Neonates; Newborn babies; Original Article; Pancreatitis; Pancreatitis-Associated Proteins; Pediatrics; Prenatal development; Prevention and actions; Public health. Hygiene; Public health. Hygiene-occupational medicine; Sex Factors
• ISSN: 0141-8955; 1573-2665
• DOI: 10.1007/s10545-012-9498-6

Background Pancreatitis-associated protein (PAP) is currently discussed as a marker in newborn screening (NBS) for cystic fibrosis (CF). However, it is not known if PAP concentrations are influenced by sex, gestational age, birth weight, blood transfusion or time of collection and what this would mean for NBS for CF. Methods In 2008 all newborns in part of the Netherlands were screened for CF by an IRT/PAP protocol. PAP concentration was determined by the MucoPAP ELISA (DynaBio), which was modified to a Dissociation Enhanced Lanthanide Fluoroimmunoassay (DELFIA) method following a protocol of PerkinElmer. Results In healthy newborns, the median PAP concentration was 0.5 μg/l (Interquartile range (IQR 0.3-0.8) whereas this was 3.2 μg/l (IQR 2.0-12.5) in CF infants. PAP concentrations were lower in premature infants 0.94 and 0.91 times for 25 to 31 + 6 weeks GA and 32 to 36 + 6 weeks respectively. A higher PAP concentration was observed in low-birth-weight infants (