Characterization of the cardiac ganglion in the crab Neohelice granulata and immunohistochemical evidence of GABA-like extrinsic regulation

• Published in: Arthropod structure & development 2013-01, Vol.42 (1), p.17-25
• Main Authors: Yang, Margarita, Carbó Tano, Martín, Freudenthal, Ramiro, Hermitte, Gabriela
• Format: Article
• Language: English
• Subjects: Animals; Argentina; Arthropoda; Brachyura - cytology; Brachyura - physiology; Brachyura - ultrastructure; Cardiac ganglion; Cardiac inhibitory response; crabs; Decapoda; Decapods; Extrinsic regulation; GABAergic system; gamma-aminobutyric acid; gamma-Aminobutyric Acid - metabolism; ganglia; Ganglia, Sympathetic - cytology; Ganglia, Sympathetic - ultrastructure; heart; immunohistochemistry; Microscopy, Confocal; motor neurons; Motor Neurons - cytology; Motor Neurons - ultrastructure; Myocardium - cytology; Myocardium - ultrastructure
• ISSN: 1467-8039; 1873-5495
• DOI: 10.1016/j.asd.2012.09.002

The aim of the present work is to provide an anatomical description of the cardiac system in the crab Neohelice granulata and evidence of the presence of GABA by means of immunohistochemistry. The ganglionic trunk was found lying on the inner surface of the heart's dorsal wall. After dissection, this structure appeared as a Y-shaped figure with its major axis perpendicular to the major axis of the heart. Inside the cardiac ganglion, we identified four large neurons of 63.7 μm ± 3.7 in maximum diameter, which were similar to the motor neurons described in other decapods. All the GABA-like immunoreactivity (GABAi) was observed as processes entering mainly the ganglionic trunk and branching in slender varicose fibers, forming a network around the large neurons suggesting that GABAi processes contact them. Our findings strengthen previous results suggesting that the GABAergic system mediates the cardio-inhibitory response upon sensory stimulation. ► The gross morphology of the cardiac system and the ganglionic trunk is described. ► Four large ganglion cells have been identified and characterized within the cardiac ganglion. ► GABA-like immunoreactive processes were found surrounding the large neurons. ► These processes might mediate extrinsic regulation during cardioinhibitory responses.