Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae

• Published in: Antonie van Leeuwenhoek 2013-02, Vol.103 (2), p.399-408
• Main Authors: Jia, Feiyu, Liu, Chongxi, Wang, Xiangjing, Zhao, Junwei, Liu, Qianfeng, Zhang, Ji, Gao, Ruixia, Xiang, Wensheng
• Format: Article
• Language: English
• Subjects: Bacteria; Base Composition; Biomedical and Life Sciences; Carbohydrates - analysis; Cell Wall - chemistry; Cluster Analysis; Cytosol - chemistry; Diaminopimelic Acid - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Endophytes - classification; Endophytes - genetics; Endophytes - isolation & purification; Fatty Acids - analysis; Glycine max - microbiology; Life Sciences; Medical Microbiology; Microbiology; Micromonosporaceae - classification; Micromonosporaceae - genetics; Micromonosporaceae - isolation & purification; Molecular Sequence Data; Original Paper; Phospholipids - analysis; Phylogenetics; Phylogeny; Plant Roots - microbiology; Plant Sciences; Ribonucleic acid; RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Science & Conservation; Soybeans; Taxonomy; Vitamin K 2 - analysis
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-012-9820-1

A novel endophytic actinomycete, designated strain NEAU-J3 T , was isolated from soybean root ( Glycine max (L.) Merr) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain NEAU-J3 T fell within the family Micromonosporaceae . The strain was observed to form an extensively branched substrate mycelium, which carried non-motile oval spores with a smooth surface. The cell walls of strain NEAU-J3 T were determined to contain meso -diaminopimelic acid and galactose, ribose and glucose were detected as whole-cell sugars. The major menaquinones were determined to be MK-9(H 4 ) and MK-9(H 6 ). The phospholipids detected were phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were determined to be C 16:0 , C 18:1 ω9c, C 18:0 , C 17:0 , C 17:1 ω7c, anteiso-C 17:0 , C 16:1 ω7c and C 15:0 . The DNA G + C content was 62.5 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J3 T is considered to represent a novel species of a new genus within the family Micromonosporaceae , for which the name Wangella harbinensis gen. nov., sp. nov. is proposed. The type strain of Wangella harbinensis is strain NEAU-J3 T (=CGMCC 4.7039 T  = DSM 45747 T ).