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Decrease in circulating autotaxin by oral administration of prednisolone
Autotaxin (ATX), secreted mainly from adipose tissue, functions as a lysophospholipase D (lysoPLD) to hydrolyze lysophosphatidylcholine (LPC) into lysophosphatidic acid (LPA). ATX–LPA signaling is implicated in a wide range of physiological and pathophysiological processes including immune response....
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Published in: | Clinica chimica acta 2013-01, Vol.415, p.74-80 |
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Main Authors: | , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Autotaxin (ATX), secreted mainly from adipose tissue, functions as a lysophospholipase D (lysoPLD) to hydrolyze lysophosphatidylcholine (LPC) into lysophosphatidic acid (LPA). ATX–LPA signaling is implicated in a wide range of physiological and pathophysiological processes including immune response.
The present study measured serum ATX antigen levels in patients with various autoimmune diseases using a recently developed automated enzyme immunoassay. In addition, serum lysoPLD activity was assessed by measuring choline liberation from the substrate LPC. Moreover, the effect of prednisolone (PSL) on mRNA expression of ATX was evaluated using cultured adipose tissue from mice.
Decreased serum ATX antigen levels were observed after the initiation of treatment with PSL. The decreased levels recovered during tapering of PSL dose in a dose-dependent manner without exacerbation of disease activity. Moreover, decreased ATX mRNA expression in PSL-treated cultured murine adipose tissue suggested that the effect of PSL on serum ATX may have resulted from changes in adipose tissue ATX expression.
Our results suggest that measurement of serum ATX antigen level may be clinically useful for the assessment of steroid treatment effect and drug compliance with steroids. Furthermore, our findings provide many novel insights into the biosynthesis, physiological functions, pathological roles, and clinical significance of circulating ATX.
► ATX levels were measured in autoimmune disease patients before and after PSL therapy. ► ATX levels in serum of patients showed an inverse correlation with PSL dosage. ► During tapering of PSL dosage, ATX levels increased, independent of disease activity. ► Decreased ATX levels during PSL treatment correlated with decreased lysoPLD activity. ► PSL treatment decreased ATX mRNA levels in cultured murine adipose tissue. |
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ISSN: | 0009-8981 1873-3492 |
DOI: | 10.1016/j.cca.2012.10.003 |