Distribution of H2S Synthesis Enzymes in the Walls of Cerebral Arteries in Rats

• Published in: Bulletin of experimental biology and medicine 2012-11, Vol.154 (1), p.104-107
• Main Authors: Chertok, V. M., Kotsyuba, A. E.
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Carotid Artery, Internal - metabolism; Cell Biology; Cerebral Arteries - enzymology; Cerebral Arteries - innervation; Cerebral Arteries - metabolism; Cystathionine beta-Synthase - metabolism; Cystathionine gamma-Lyase - metabolism; Endothelium, Vascular - metabolism; Hydrogen Sulfide - metabolism; Immunohistochemistry; Internal Medicine; Laboratory Medicine; Male; Middle Cerebral Artery - metabolism; Pathology; Rats; Rats, Wistar; Tunica Media - metabolism
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-012-1886-2

The distribution of two enzymes involved in H 2 S synthesis, cystationine β-synthase (CBS) and cystationine γ-liase (CSE), was studied in the walls of the internal carotid artery, order I-V branches of the middle cerebral artery basin, and intracerebral vessels of adult Wistar rats. Immunohistochemical staining showed the presence of CBS in the endothelium of small pial arteries (order IV-V branches) and intracerebral arterioles and in the capillary walls, neurons, and vascular nerves. As for CSE, in the internal carotid artery and large (order I-II) pial branches it was found mainly in the tunica media myocytes, in order III-IV vessels in myocytes and endothelium, and in smaller pial and intracerebral vessels in the endothelium. Along with enzyme-positive vessels, many pial and intracerebral arteries contained no these enzymes in the walls.