Prevalence and molecular characterization of Enterobacteriaceae producing NDM-1 carbapenemase at a military hospital in Pakistan and evaluation of two chromogenic media

Abstract The aim of this study was to assess the frequency and genotypic diversity of carbapenemase-producing Enterobacteriaceae (CPE) in stool samples from patients attending a military hospital in Pakistan. Further aims included the identification of factors that might predispose to faecal carriag...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2013-02, Vol.75 (2), p.187-191
Main Authors: Day, Kathryn M, Ali, Shamshad, Mirza, Irfan Ali, Sidjabat, Hanna E, Silvey, Anna, Lanyon, Clare V, Cummings, Stephen P, Abbasi, Shahid Ahmed, Raza, Muhammad W, Paterson, David L, Perry, John D
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Antimicrobial resistance
beta-Lactam Resistance
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Carbapenemase
Child
Culture Media
Enterobacteriaceae
Enterobacteriaceae - drug effects
Enterobacteriaceae - enzymology
Enterobacteriaceae - genetics
Enterobacteriaceae - isolation & purification
Enterobacteriaceae Infections - microbiology
Feces - microbiology
Genes, Bacterial
Hospitals, Military
Humans
Infectious Disease
Internal Medicine
Length of Stay
Male
Microbial Sensitivity Tests
Middle Aged
Phylogeny
Prevalence
β-Lactamase
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Summary:Abstract The aim of this study was to assess the frequency and genotypic diversity of carbapenemase-producing Enterobacteriaceae (CPE) in stool samples from patients attending a military hospital in Pakistan. Further aims included the identification of factors that might predispose to faecal carriage and evaluation of 2 chromogenic culture media: Brilliance CRE and chromID CARBA. Of 175 patients, 32 (18.3%) had faecal carriage of CPE and all produced NDM-1 carbapenemase. All of these 32 patients were detected using chromID CARBA compared with 20 patients (62.5%) detected using Brilliance CRE ( P = 0.0015). Duration of hospitalization and treatment with co-amoxyclav were statistically associated with a higher likelihood of carriage of CPE ( P ≤ 0.05). The majority of NDM-1–producing Enterobacteriaceae co-produced CTX-M-1 group extended spectrum β-lactamase, and one third produced armA- type methylase. NDM-1 carbapenemase was most commonly found amongst commensal types of Escherichia coli , especially phylogenetic group B1.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2012.11.006