S100A10 is required for the organization of actin stress fibers and promotion of cell spreading

Dynamic remodeling of the actin cytoskeleton is crucial for biological processes such as cell migration and cell spreading. S100A10 is a member of the S100 protein family and is involved in intracellular trafficking and cell migration. In this study, we examined the role of S100A10 in actin cytoskel...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2013-02, Vol.374 (1-2), p.105-111
Main Authors: Sayeed, Shurovi, Asano, Eri, Ito, Satoko, Ohno, Kinji, Hamaguchi, Michinari, Senga, Takeshi
Format: Article
Language:English
Subjects:
Actin
Actin Cytoskeleton - metabolism
Annexin A2 - genetics
Annexin A2 - metabolism
Biochemistry
Biomedical and Life Sciences
Cardiology
Cell Adhesion
Cell adhesion & migration
Cell Line, Tumor
Cell Movement
Cytoskeleton
Fibers
Gene expression
HEK293 Cells
HeLa Cells
Humans
Life Sciences
Medical Biochemistry
Muscle proteins
Oncology
Protein Transport
Proteins
rac1 GTP-Binding Protein - metabolism
RNA Interference
RNA, Small Interfering
S100 Proteins - genetics
S100 Proteins - metabolism
Stress Fibers
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Summary:Dynamic remodeling of the actin cytoskeleton is crucial for biological processes such as cell migration and cell spreading. S100A10 is a member of the S100 protein family and is involved in intracellular trafficking and cell migration. In this study, we examined the role of S100A10 in actin cytoskeletal organization and cell spreading. Depletion of S100A10 induced disruption of stress fiber formation and delay in cell spreading. Rac1 activation during spreading was suppressed by S100A10 knockdown, and exogenous expression of active Rac1 restored the ability of cells to spread in the absence of S100A10. Our results demonstrate the crucial role of S100A10 in actin dynamics promoting cell spreading via Rac1 activation.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-012-1509-2