Postconditioning with far-infrared irradiation increases heme oxygenase-1 expression and protects against ischemia/reperfusion injury in rat testis

Studies have shown that heme oxygenase-1 (HO-1) has a protective role in the mechanism underlying hypoxic preconditioning. We used a far-infrared radiation (FIR) heater to investigate the postconditioning protective role of HO-1 against ischemia/reperfusion (I/R) injury in rat testis. Forty rats wer...

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Bibliographic Details
Published in:Life sciences (1973) 2013-01, Vol.92 (1), p.35-41
Main Authors: Tu, Yuan-Po, Chen, Shyh-Chyan, Liu, Yi-Hsia, Chen, Chau-Fong, Hour, Tzyh-Chyuan
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - radiation effects
Blotting, Western
Caspase 3 - metabolism
caspase-3
Far-infrared radiation
Gene Expression Regulation - radiation effects
heat
heme oxygenase (biliverdin-producing)
Heme oxygenase-1
Heme Oxygenase-1 - genetics
Hot Temperature
Immunohistochemistry
In Situ Nick-End Labeling
Infrared Rays
irradiation
ischemia
Ischemia/reperfusion
Ischemic Postconditioning - methods
Light
Male
Postconditioning
protective effect
protein synthesis
Rat testis
Rats
Rats, Wistar
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
testes
Testis - blood supply
Testis - pathology
Time Factors
tissues
Western blotting
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Summary:Studies have shown that heme oxygenase-1 (HO-1) has a protective role in the mechanism underlying hypoxic preconditioning. We used a far-infrared radiation (FIR) heater to investigate the postconditioning protective role of HO-1 against ischemia/reperfusion (I/R) injury in rat testis. Forty rats were used. Testis ischemia was mimicked by total obstructive clamping of testis vessels for 1, 2, or 4h, and concomitant postconditioning with 30min FIR or heat light during initially 30min reperfusion. HO-1 expression and apoptosis of testis tissues were examined by immunohistochemistry and in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay, respectively. HO-1 protein level and caspase-3 activity were analyzed by Western blotting. There was less apoptotic activity in rat testis after FIR, as determined by TUNEL assay. Higher HO-1 protein expression was observed by immunohistochemistry and Western blotting (p
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2012.10.019