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OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity
Background The interaction between the OX40 ligand (OX40L) and OX40 has been suggested to have pathogenetic significance in atopic dermatitis (AD). Objective The purpose of this study was to investigate the expression and relevance of OX40L and OX40 in AD skin. Methods OX40L and OX40 were stained...
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| Published in: | Journal of the European Academy of Dermatology and Venereology 2013-02, Vol.27 (2), p.e197-e205 |
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| container_title | Journal of the European Academy of Dermatology and Venereology |
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| creator | Ilves, T. Harvima, I.T. |
| description | Background The interaction between the OX40 ligand (OX40L) and OX40 has been suggested to have pathogenetic significance in atopic dermatitis (AD).
Objective The purpose of this study was to investigate the expression and relevance of OX40L and OX40 in AD skin.
Methods OX40L and OX40 were stained immunohistochemically on the cryosections of the lesional and non‐lesional skin of 17 subjects with moderate‐to‐severe AD and of 10 patients with psoriasis vulgaris. Phorbol myristate acetate (PMA) stimulated keratinocytes and cell membrane preparations from PMA‐stimulated keratinocytes or LAD‐2 mast cells were incubated with peripheral blood mononuclear cells (PBMC) in the presence or absence of blocking monoclonal antibodies to OX40L, CD30L or ICAM‐1.
Results We show for the first time that the staining intensity of OX40L and the number of OX40+ cells are significantly greater in the lesional dermis than in the healthy‐looking dermis in AD (P |
| doi_str_mv | 10.1111/j.1468-3083.2012.04587.x |
| format | article |
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Objective The purpose of this study was to investigate the expression and relevance of OX40L and OX40 in AD skin.
Methods OX40L and OX40 were stained immunohistochemically on the cryosections of the lesional and non‐lesional skin of 17 subjects with moderate‐to‐severe AD and of 10 patients with psoriasis vulgaris. Phorbol myristate acetate (PMA) stimulated keratinocytes and cell membrane preparations from PMA‐stimulated keratinocytes or LAD‐2 mast cells were incubated with peripheral blood mononuclear cells (PBMC) in the presence or absence of blocking monoclonal antibodies to OX40L, CD30L or ICAM‐1.
Results We show for the first time that the staining intensity of OX40L and the number of OX40+ cells are significantly greater in the lesional dermis than in the healthy‐looking dermis in AD (P < 0.001 in both comparisons) and also in psoriasis (P = 0.01 and P < 0.001 respectively), but neither molecule correlate significantly with the clinical severity of AD. Living keratinocytes and cell membranes from LAD‐2 mast cells and keratinocytes increased the PBMC proliferation response. Anti‐OX40L antibody inhibited, in a similar fashion as anti‐ICAM‐1 and anti‐CD30L, PBMC proliferation induced by LAD‐2 membranes, but stimulated that induced by keratinocytes.
Conclusion Our findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction.</description><identifier>ISSN: 0926-9959</identifier><identifier>EISSN: 1468-3083</identifier><identifier>DOI: 10.1111/j.1468-3083.2012.04587.x</identifier><identifier>PMID: 22646697</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Cell Proliferation ; Dermatitis, Atopic - metabolism ; Dermatitis, Atopic - pathology ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Monocytes - cytology ; OX40 Ligand - metabolism ; Receptors, OX40 - metabolism ; Severity of Illness Index ; Young Adult</subject><ispartof>Journal of the European Academy of Dermatology and Venereology, 2013-02, Vol.27 (2), p.e197-e205</ispartof><rights>2012 The Authors. Journal of the European Academy of Dermatology and Venereology © 2012 European Academy of Dermatology and Venereology</rights><rights>2012 The Authors. Journal of the European Academy of Dermatology and Venereology © 2012 European Academy of Dermatology and Venereology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4077-c44d986d066ac4cf81b5465e60a1ad02af5cfcc9e3213fa4f3ed735e514f0c183</citedby><cites>FETCH-LOGICAL-c4077-c44d986d066ac4cf81b5465e60a1ad02af5cfcc9e3213fa4f3ed735e514f0c183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22646697$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ilves, T.</creatorcontrib><creatorcontrib>Harvima, I.T.</creatorcontrib><title>OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity</title><title>Journal of the European Academy of Dermatology and Venereology</title><addtitle>J Eur Acad Dermatol Venereol</addtitle><description>Background The interaction between the OX40 ligand (OX40L) and OX40 has been suggested to have pathogenetic significance in atopic dermatitis (AD).
Objective The purpose of this study was to investigate the expression and relevance of OX40L and OX40 in AD skin.
Methods OX40L and OX40 were stained immunohistochemically on the cryosections of the lesional and non‐lesional skin of 17 subjects with moderate‐to‐severe AD and of 10 patients with psoriasis vulgaris. Phorbol myristate acetate (PMA) stimulated keratinocytes and cell membrane preparations from PMA‐stimulated keratinocytes or LAD‐2 mast cells were incubated with peripheral blood mononuclear cells (PBMC) in the presence or absence of blocking monoclonal antibodies to OX40L, CD30L or ICAM‐1.
Results We show for the first time that the staining intensity of OX40L and the number of OX40+ cells are significantly greater in the lesional dermis than in the healthy‐looking dermis in AD (P < 0.001 in both comparisons) and also in psoriasis (P = 0.01 and P < 0.001 respectively), but neither molecule correlate significantly with the clinical severity of AD. Living keratinocytes and cell membranes from LAD‐2 mast cells and keratinocytes increased the PBMC proliferation response. Anti‐OX40L antibody inhibited, in a similar fashion as anti‐ICAM‐1 and anti‐CD30L, PBMC proliferation induced by LAD‐2 membranes, but stimulated that induced by keratinocytes.
Conclusion Our findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction.</description><subject>Adult</subject><subject>Aged</subject><subject>Cell Proliferation</subject><subject>Dermatitis, Atopic - metabolism</subject><subject>Dermatitis, Atopic - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Monocytes - cytology</subject><subject>OX40 Ligand - metabolism</subject><subject>Receptors, OX40 - metabolism</subject><subject>Severity of Illness Index</subject><subject>Young Adult</subject><issn>0926-9959</issn><issn>1468-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkM1uEzEURi0EoqHlFZCXbGbw_3gWLCBACqpaCdHCznLsO-DUmUlthyZvz0xTsq4l29fyd67lgxCmpKbjeLeqqVC64kTzmhHKaiKkburdMzQ7XjxHM9IyVbWtbE_Qq5xXhBBKpX6JThhTQqm2maHbq1-C4Bh-297jaT6cbQIcepfAZvBjhW0ZNsFhD2ltSygh4wg5DH3Gy23BfsD9ULAbUoJoC-D7UP5gF0MfnI04w19IoezP0IvOxgyvH_dTdP3l84_5eXVxtfg6_3BROUGaZlyFb7XyRCnrhOs0XUqhJChiqfWE2U66zrkWOKO8s6Lj4BsuQVLREUc1P0VvD303abjbQi5mHbKDGG0PwzYbyhreNEJzNUb1IerSkHOCzmxSWNu0N5SYSbVZmcmomYyaSbV5UG12I_rm8ZXtcg3-CP53OwbeHwL3IcL-yY3Nt083UzXy1YEPucDuyNt0a9T4AWl-Xi7MR3bOvt8s5mbB_wFTFJxh</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Ilves, T.</creator><creator>Harvima, I.T.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201302</creationdate><title>OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity</title><author>Ilves, T. ; Harvima, I.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4077-c44d986d066ac4cf81b5465e60a1ad02af5cfcc9e3213fa4f3ed735e514f0c183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Cell Proliferation</topic><topic>Dermatitis, Atopic - metabolism</topic><topic>Dermatitis, Atopic - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Monocytes - cytology</topic><topic>OX40 Ligand - metabolism</topic><topic>Receptors, OX40 - metabolism</topic><topic>Severity of Illness Index</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ilves, T.</creatorcontrib><creatorcontrib>Harvima, I.T.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the European Academy of Dermatology and Venereology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ilves, T.</au><au>Harvima, I.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity</atitle><jtitle>Journal of the European Academy of Dermatology and Venereology</jtitle><addtitle>J Eur Acad Dermatol Venereol</addtitle><date>2013-02</date><risdate>2013</risdate><volume>27</volume><issue>2</issue><spage>e197</spage><epage>e205</epage><pages>e197-e205</pages><issn>0926-9959</issn><eissn>1468-3083</eissn><abstract>Background The interaction between the OX40 ligand (OX40L) and OX40 has been suggested to have pathogenetic significance in atopic dermatitis (AD).
Objective The purpose of this study was to investigate the expression and relevance of OX40L and OX40 in AD skin.
Methods OX40L and OX40 were stained immunohistochemically on the cryosections of the lesional and non‐lesional skin of 17 subjects with moderate‐to‐severe AD and of 10 patients with psoriasis vulgaris. Phorbol myristate acetate (PMA) stimulated keratinocytes and cell membrane preparations from PMA‐stimulated keratinocytes or LAD‐2 mast cells were incubated with peripheral blood mononuclear cells (PBMC) in the presence or absence of blocking monoclonal antibodies to OX40L, CD30L or ICAM‐1.
Results We show for the first time that the staining intensity of OX40L and the number of OX40+ cells are significantly greater in the lesional dermis than in the healthy‐looking dermis in AD (P < 0.001 in both comparisons) and also in psoriasis (P = 0.01 and P < 0.001 respectively), but neither molecule correlate significantly with the clinical severity of AD. Living keratinocytes and cell membranes from LAD‐2 mast cells and keratinocytes increased the PBMC proliferation response. Anti‐OX40L antibody inhibited, in a similar fashion as anti‐ICAM‐1 and anti‐CD30L, PBMC proliferation induced by LAD‐2 membranes, but stimulated that induced by keratinocytes.
Conclusion Our findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22646697</pmid><doi>10.1111/j.1468-3083.2012.04587.x</doi><tpages>9</tpages></addata></record> |
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| source | Wiley:Jisc Collections:Wiley Read and Publish Open Access 2024-2025 (reading list) |
| subjects | Adult Aged Cell Proliferation Dermatitis, Atopic - metabolism Dermatitis, Atopic - pathology Female Humans Immunohistochemistry Male Middle Aged Monocytes - cytology OX40 Ligand - metabolism Receptors, OX40 - metabolism Severity of Illness Index Young Adult |
| title | OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity |
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