Bovine mitochondrial oxygen consumption effects on oxymyoglobin in the presence of lactate as a substrate for respiration

• Published in: Meat science 2013-04, Vol.93 (4), p.893-897
• Main Authors: Ramanathan, R., Mancini, R.A., Joseph, P., Suman, S.P.
• Format: Article
• Language: English
• Subjects: Animals; antimycin A; Antimycin A - pharmacology; beef; Beef color; Beef darkening; Cattle; Cell Respiration; L-Lactate Dehydrogenase - metabolism; L-Lactate Dehydrogenase - pharmacology; lactates; Lactic Acid - metabolism; Lactic Acid - pharmacology; Meat; mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondria, Heart - drug effects; Mitochondria, Heart - metabolism; Muscle, Skeletal - metabolism; Muscles - drug effects; Muscles - metabolism; myocardium; Myocardium - metabolism; Myoglobin; Myoglobin - metabolism; NAD (coenzyme); NAD - metabolism; NAD - pharmacology; Oxidation-Reduction; Oxygen Consumption
• ISSN: 0309-1740; 1873-4138
• DOI: 10.1016/j.meatsci.2012.12.005

Research suggests that NADH formed from lactate addition can increase mitochondrial oxygen consumption. However, limited research has assessed the subsequent effects of lactate-mediated mitochondrial oxygen consumption on oxymyoglobin. Therefore, our objective was to assess the effects of bovine mitochondrial oxygen consumption on oxymyoglobin in the presence of lactate, LDH, and NAD. Isolated beef cardiac and skeletal muscle mitochondria (n=5) were reacted with lactate (40mM), LDH (100units), and NAD (0.02mM) to initiate oxygen consumption. Myoglobin redox state was measured to assess the effects of oxygen consumption on oxymyoglobin. The addition of lactate–LDH–NAD to mitochondria increased (p