Rapid and Simple Method for the Determination of Emodin in Tartary Buckwheat (Fagopyrum tataricum) by High-Performance Liquid Chromatography Coupled to a Diode Array Detector

• Published in: Journal of agricultural and food chemistry 2013-01, Vol.61 (4), p.854-857
• Main Authors: Peng, Lian-Xin, Wang, Jing-Bo, Hu, Li-Xue, Zhao, Jiang-Lin, Xiang, Da-Bing, Zou, Liang, Zhao, Gang
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; buckwheat; Cereal and baking product industries; Chromatography, High Pressure Liquid - methods; correlation; detection limit; emodin; Emodin - analysis; Fagopyrum - chemistry; Fagopyrum tataricum; Food industries; Fundamental and applied biological sciences. Psychology; high performance liquid chromatography; Plant Extracts - chemistry; rapid methods; Reproducibility of Results; Seeds - chemistry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf304804c

A simple and rapid method for determining emodin, an active factor presented in tartary buckwheat (Fagopyrum tataricum), by high-performance liquid chromatography coupled to a diode array detector (HPLC–DAD) has been developed. Emodin was separated from an extract of buckwheat on a Kromasil-ODS C18 (250 mm × 4.6 mm × 5 μm) column. The separation is achieved within 15 min on the ODS column. Emodin can be quantified using an external standard method detecting at 436 nm. Good linearity is obtained with a correlation coefficient exceeding 0.9992. The limit of detection and the limit of quantification are 5.7 and 19 μg/L, respectively. This method shows good reproducibility for the quantification of the emodin with a relative standard deviation value of 4.3%. Under optimized extraction conditions, the recovery of emodin was calculated as >90%. The validated method is successfully applied to quantify the emodin in tartary buckwheat and its products.