Determination of gadolinium-based magnetic resonance imaging contrast agents by micellar electrokinetic capillary chromatography

MEKC with DAD was applied to detect six Gd‐based contrasting agents (CAs) (Gd‐DTPA‐BMA (Omniscan), Gd‐HPDO3A (ProHance), Gd‐DOTA (Dotarem), Gd‐AAZTA, Gd‐BOPTA (Multihance) and Gd‐DTPA (Magnevist)) commonly used in MRI diagnostics. The achieved LODs ranged between 0.40 and 20 μM and the optimized met...

Full description

Saved in:
Bibliographic Details
Published in:Electrophoresis 2011-08, Vol.32 (16), p.2223-2228
Main Authors: Andrási, Melinda, Gáspár, Attila, Kovács, Otilia, Baranyai, Zsolt, Klekner, Álmos, Brücher, Ernő
Format: Article
Language:English
Subjects:
Chromatography, Micellar Electrokinetic Capillary - methods
Contrast agents
Contrast Media - analysis
Contrast Media - chemistry
Drug Monitoring
Gadolinium
Gadolinium - blood
Gadolinium - chemistry
Gadolinium - urine
Humans
Limit of Detection
Linear Models
Magnetic Resonance Imaging - methods
MEKC
Organometallic Compounds - blood
Organometallic Compounds - chemistry
Organometallic Compounds - urine
Reproducibility of Results
Serum
Sodium Dodecyl Sulfate - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MEKC with DAD was applied to detect six Gd‐based contrasting agents (CAs) (Gd‐DTPA‐BMA (Omniscan), Gd‐HPDO3A (ProHance), Gd‐DOTA (Dotarem), Gd‐AAZTA, Gd‐BOPTA (Multihance) and Gd‐DTPA (Magnevist)) commonly used in MRI diagnostics. The achieved LODs ranged between 0.40 and 20 μM and the optimized method gave excellent precision, especially when two internal standards were applied (less than 0.34 RSD% for migration time). The MEKC technique made it possible to determine the CAs in urine and serum samples of patients having a therapeutic dose. Due to the SDS content of the running buffer, the serum samples can be directly injected to analyze Gd‐based CAs without interference of high protein content.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201100185