Dehydration Method for Immature or Wet Cotton Fibers for Light and Electron Microscopy

A rapid dehydration procedure with acidified 2,2-dimethoxypropane (DMP) has been successfully applied to immature or wet cotton fibers. The ultrastructural integrity and fiber quality parameters such as cell wall area and perimeter of developing and mature cotton fibers were well-pre-served using th...

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Bibliographic Details
Published in:Textile research journal 2006-06, Vol.76 (6), p.514-518
Main Authors: Rajasekaran, K, Muir, A.J, French, A.D
Format: Article
Language:English
Subjects:
Acidification
Cotton fibers
Cross sections
Dehydration
Drying
Fibers
Gossypium hirsutum
lint cotton
scanning electron microscopes
Scanning electron microscopy
transmission electron microscopes
ultrastructure
Walls
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Summary:A rapid dehydration procedure with acidified 2,2-dimethoxypropane (DMP) has been successfully applied to immature or wet cotton fibers. The ultrastructural integrity and fiber quality parameters such as cell wall area and perimeter of developing and mature cotton fibers were well-pre-served using this procedure as demonstrated by light, scanning and electron microscopy of whole fibers and cross-sections. Embedding in resin following DMP dehydration also prevented the collapse of the immature fibers due to drying of lumen.
ISSN:0040-5175
1746-7748
DOI:10.1177/0040517506063390