Confocal line-scanning microscope with modified illumination

A modified illumination-based method has been proposed to improve resolution of a confocal line-scanning system by 20%. Phase-only apodization is applied to the illumination and combined with confocal detection. The method was studied both theoretically and experimentally. Measurements were performe...

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Bibliographic Details
Published in:Optics letters 2012-10, Vol.37 (20), p.4293-4295
Main Authors: Dudas, L, Sinko, J, Erdelyi, M, Szabo, G
Format: Article
Language:English
Subjects:
Apodization
Confocal
Illumination
Microscopes
Modulation transfer function
Nanospheres
Silver
Spatial resolution
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Summary:A modified illumination-based method has been proposed to improve resolution of a confocal line-scanning system by 20%. Phase-only apodization is applied to the illumination and combined with confocal detection. The method was studied both theoretically and experimentally. Measurements were performed on silver nanospheres as subresolution test samples, and the captured data were analyzed to determine the modulation transfer function and ultimately the spatial resolution of the system.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.37.004293