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UV photoactivation of 7-dehydrocholesterol on titanium implants enhances osteoblast differentiation and decreases Rankl gene expression

Vitamin D plays a central role in bone regeneration, and its insufficiency has been reported to have profound negative effects on implant osseointegration. The present study aimed to test the in vitro biological effect of titanium (Ti) implants coated with UV-activated 7-dehydrocholesterol (7-DHC),...

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Published in:Acta biomaterialia 2013-03, Vol.9 (3), p.5759-5770
Main Authors: Satué, M., Petzold, C., Córdoba, A., Ramis, J.M., Monjo, M.
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description Vitamin D plays a central role in bone regeneration, and its insufficiency has been reported to have profound negative effects on implant osseointegration. The present study aimed to test the in vitro biological effect of titanium (Ti) implants coated with UV-activated 7-dehydrocholesterol (7-DHC), the precursor of vitamin D, on cytotoxicity and osteoblast differentiation. Fourier transform infrared spectroscopy confirmed the changes in chemical structure of 7-DHC after UV exposure. High-pressure liquid chromatography analysis determined a 16.5±0.9% conversion of 7-DHC to previtamin D3 after 15min of UV exposure, and a 34.2±4.8% of the preD3 produced was finally converted to 25-hydroxyvitamin D3 (25-D3) by the osteoblastic cells. No cytotoxic effect was found for Ti implants treated with 7-DHC and UV-irradiated. Moreover, Ti implants treated with 7-DHC and UV-irradiated for 15min showed increased 25-D3 production, together with increased ALP activity and calcium content. Interestingly, Rankl gene expression was significantly reduced in osteoblasts cultured on 7-DHC-coated Ti surfaces when UV-irradiated for 15 and 30min to 33.56±15.28% and 28.21±4.40%, respectively, compared with the control. In conclusion, these findings demonstrate that UV-activated 7-DHC is a biocompatible coating of Ti implants, which allows the osteoblastic cells to produce themselves active vitamin D, with demonstrated positive effects on osteoblast differentiation in vitro.
doi_str_mv 10.1016/j.actbio.2012.11.021
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The present study aimed to test the in vitro biological effect of titanium (Ti) implants coated with UV-activated 7-dehydrocholesterol (7-DHC), the precursor of vitamin D, on cytotoxicity and osteoblast differentiation. Fourier transform infrared spectroscopy confirmed the changes in chemical structure of 7-DHC after UV exposure. High-pressure liquid chromatography analysis determined a 16.5±0.9% conversion of 7-DHC to previtamin D3 after 15min of UV exposure, and a 34.2±4.8% of the preD3 produced was finally converted to 25-hydroxyvitamin D3 (25-D3) by the osteoblastic cells. No cytotoxic effect was found for Ti implants treated with 7-DHC and UV-irradiated. Moreover, Ti implants treated with 7-DHC and UV-irradiated for 15min showed increased 25-D3 production, together with increased ALP activity and calcium content. Interestingly, Rankl gene expression was significantly reduced in osteoblasts cultured on 7-DHC-coated Ti surfaces when UV-irradiated for 15 and 30min to 33.56±15.28% and 28.21±4.40%, respectively, compared with the control. 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The present study aimed to test the in vitro biological effect of titanium (Ti) implants coated with UV-activated 7-dehydrocholesterol (7-DHC), the precursor of vitamin D, on cytotoxicity and osteoblast differentiation. Fourier transform infrared spectroscopy confirmed the changes in chemical structure of 7-DHC after UV exposure. High-pressure liquid chromatography analysis determined a 16.5±0.9% conversion of 7-DHC to previtamin D3 after 15min of UV exposure, and a 34.2±4.8% of the preD3 produced was finally converted to 25-hydroxyvitamin D3 (25-D3) by the osteoblastic cells. No cytotoxic effect was found for Ti implants treated with 7-DHC and UV-irradiated. Moreover, Ti implants treated with 7-DHC and UV-irradiated for 15min showed increased 25-D3 production, together with increased ALP activity and calcium content. Interestingly, Rankl gene expression was significantly reduced in osteoblasts cultured on 7-DHC-coated Ti surfaces when UV-irradiated for 15 and 30min to 33.56±15.28% and 28.21±4.40%, respectively, compared with the control. 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The present study aimed to test the in vitro biological effect of titanium (Ti) implants coated with UV-activated 7-dehydrocholesterol (7-DHC), the precursor of vitamin D, on cytotoxicity and osteoblast differentiation. Fourier transform infrared spectroscopy confirmed the changes in chemical structure of 7-DHC after UV exposure. High-pressure liquid chromatography analysis determined a 16.5±0.9% conversion of 7-DHC to previtamin D3 after 15min of UV exposure, and a 34.2±4.8% of the preD3 produced was finally converted to 25-hydroxyvitamin D3 (25-D3) by the osteoblastic cells. No cytotoxic effect was found for Ti implants treated with 7-DHC and UV-irradiated. Moreover, Ti implants treated with 7-DHC and UV-irradiated for 15min showed increased 25-D3 production, together with increased ALP activity and calcium content. Interestingly, Rankl gene expression was significantly reduced in osteoblasts cultured on 7-DHC-coated Ti surfaces when UV-irradiated for 15 and 30min to 33.56±15.28% and 28.21±4.40%, respectively, compared with the control. In conclusion, these findings demonstrate that UV-activated 7-DHC is a biocompatible coating of Ti implants, which allows the osteoblastic cells to produce themselves active vitamin D, with demonstrated positive effects on osteoblast differentiation in vitro.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23201015</pmid><doi>10.1016/j.actbio.2012.11.021</doi><tpages>12</tpages></addata></record>
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subjects 7-Dehydrocholesterol
Alkaline Phosphatase - metabolism
Animals
Calcification, Physiologic - drug effects
Calcification, Physiologic - genetics
calcium
Cell Count
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cell Line
Cell Shape - drug effects
Cell Survival - drug effects
chemical structure
Cholecalciferol - pharmacology
Coated Materials, Biocompatible - pharmacology
coatings
cytotoxicity
Dehydrocholesterols - radiation effects
Fourier transform infrared spectroscopy
gene expression
Gene Expression Regulation - drug effects
Gene Expression Regulation - radiation effects
high performance liquid chromatography
Mice
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
Osteoblast differentiation
osteoblasts
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - enzymology
Osteoblasts - radiation effects
Prostheses and Implants
RANK Ligand - genetics
RANK Ligand - metabolism
Spectroscopy, Fourier Transform Infrared
Time Factors
titanium
Titanium - pharmacology
Titanium implant
ultraviolet radiation
Ultraviolet Rays
UV irradiation
Vitamin D
title UV photoactivation of 7-dehydrocholesterol on titanium implants enhances osteoblast differentiation and decreases Rankl gene expression
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