Viable circulating tumor cell enrichment by flexible micro spring array

We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. T...

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Bibliographic Details
Published in:2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society 2012-01, Vol.2012, p.6269-6272
Main Authors: Harouaka, R. A., Ming-Da Zhou, Yeh, T. Y., Khan, W. J., Allerton, J., Si-Yang Zheng
Format: Article
Language:English
Subjects:
Blood
Cells (biology)
Electron tubes
Metastasis
Springs
Tumors
Online Access:Request full text
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Summary:We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 10 4 enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.
ISSN:1094-687X
1558-4615
2694-0604
DOI:10.1109/EMBC.2012.6347427