Reduced protein oxidation in Wistar rats supplemented with marine ω3 PUFAs

The potential effects of various dietary eicosapentaenoic acid (EPA; 20:5) and docosahexaenoic acid (DHA; 22:6) ratios (1:1, 2:1, and 1:2, respectively) on protein redox states from plasma, kidney, skeletal muscle, and liver were investigated in Wistar rats. Dietary fish oil groups were compared wit...

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Published in:Free radical biology & medicine 2013-02, Vol.55, p.8-20
Main Authors: Méndez, Lucía, Pazos, Manuel, Gallardo, José M., Torres, Josep L., Pérez-Jiménez, Jara, Nogués, Rosa, Romeu, Marta, Medina, Isabel
Format: Article
Language:English
Subjects:
actin
albumins
alpha-linolenic acid
Animals
antioxidant activity
blood proteins
Dietary intervention
Dietary Supplements
Docosahexaenoic acid
Docosahexaenoic Acids - pharmacology
Eicosapentaenoic acid
Eicosapentaenoic Acid - pharmacology
erythrocytes
fatty acid composition
Female
Fish oil
fish oils
fluorescein
free fatty acids
Free radicals
gels
kidneys
Linoleic acid
Linolenic acid
linseed
Linseed Oil - pharmacology
liver
nutritional intervention
oxidation
Oxidation-Reduction - drug effects
Oxidative stress
protective effect
Protein carbonylation
Proteins - chemistry
Proteins - metabolism
proteomics
Rats
Rats, Wistar
skeletal muscle
Soybean Oil - pharmacology
soybeans
vegetable oil
Wistar rats
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Summary:The potential effects of various dietary eicosapentaenoic acid (EPA; 20:5) and docosahexaenoic acid (DHA; 22:6) ratios (1:1, 2:1, and 1:2, respectively) on protein redox states from plasma, kidney, skeletal muscle, and liver were investigated in Wistar rats. Dietary fish oil groups were compared with animals fed soybean and linseed oils, vegetable oils enriched in ω6 linoleic acid (LA; 18:2) and ω3 α-linolenic acid (ALA; 18:3), respectively. Fish oil treatments were effective at reducing the level of total fatty acids in plasma and enriching the plasmatic free fatty acid fraction and erythrocyte membranes in EPA and DHA. A proteomic approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labeling of protein carbonyls, FTSC intensity visualization on 1-DE or 2-DE gels, and protein identification by MS/MS was used for the protein oxidation assessment. Albumin was found to be the most carbonylated protein in plasma for all dietary groups, and its oxidation level was significantly modulated by dietary interventions. Supplementation with an equal EPA:DHA ratio (1:1) showed the lowest oxidation score for plasma albumin, followed in increasing order of carbonylation by 1:2
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2012.11.004