Luman recruiting factor regulates endoplasmic reticulum stress in mouse ovarian granulosa cell apoptosis

Follicular atresia is primarily induced by granulosa cell apoptosis; however, the molecular mechanisms that control apoptotic cell death in granulosa cells remain poorly understood. The present studies were undertaken to investigate the role of a novel endoplasmic reticulum stress-regulated gene Lum...

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Bibliographic Details
Published in:Theriogenology 2013-03, Vol.79 (4), p.633-639
Main Authors: Yang, Yanzhou, Lin, Pengfei, Chen, Fenglei, Wang, Aihua, Lan, Xiangli, Song, Yujie, Jin, Yaping
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - physiology
Caspase 12 - genetics
caspase-12
Cyclic AMP Response Element-Binding Protein - analysis
Cyclic AMP Response Element-Binding Protein - physiology
Cytoplasm - chemistry
endoplasmic reticulum
Endoplasmic reticulum stress
Endoplasmic Reticulum Stress - physiology
Female
Follicular atresia
Follicular Atresia - physiology
genes
Granulosa cell apoptosis
granulosa cells
Granulosa Cells - drug effects
Granulosa Cells - physiology
Granulosa Cells - ultrastructure
Heat-Shock Proteins - genetics
Immunohistochemistry
Luman recruiting factor
messenger RNA
Mice
Microscopy, Confocal
proteins
RNA, Messenger - analysis
Thapsigargin - pharmacology
Tunicamycin - pharmacology
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Summary:Follicular atresia is primarily induced by granulosa cell apoptosis; however, the molecular mechanisms that control apoptotic cell death in granulosa cells remain poorly understood. The present studies were undertaken to investigate the role of a novel endoplasmic reticulum stress-regulated gene Luman recruiting factor (LRF) in granulosa cell apoptosis during mouse follicular atresia. Based on immunohistochemistry and confocal laser scanning microscope analysis, LRF protein was localized in the cytoplasm of apoptotic granulosa cells, similar to localization of the LRF, Luman, CCAAT/enhancer-binding protein homologous protein and caspase-12 proteins were localized in apoptotic granulosa cells. However, glucose-regulated protein 78 protein was only present in healthy cells of the mural granulosa cell layers. A spontaneous onset of apoptotic cell death of granulosa cells was induced by thapsigargin or tunicamycin treatment in vitro, which was closely related to the increase of LRF, Luman, CCAAT/enhancer-binding protein homologous protein, and caspase-12 mRNA. Taken together, LRF might be involved in inducing apoptosis of granulosa cells through the endoplasmic reticulum stress pathway and might have a key role in mouse follicular selection.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2012.11.017