Expanded protein expression profile of human placenta using two-dimensional gel electrophoresis

• Published in: Placenta (Eastbourne) 2013-02, Vol.34 (2), p.193-196
• Main Authors: Mushahary, D, Gautam, P, Sundaram, C.S, Sirdeshmukh, R
• Format: Article
• Language: English
• Subjects: 2-D gel; Biological and medical sciences; Databases, Protein; Electrophoresis, Gel, Two-Dimensional; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; Humans; Internal Medicine; Mass spectrometry; Obstetrics and Gynecology; Placenta; Placenta - metabolism; Pregnancy; Pregnancy Proteins - isolation & purification; Pregnancy Proteins - metabolism; Pregnancy. Parturition. Lactation; Protein Array Analysis - methods; Proteomics; Tandem Mass Spectrometry; Vertebrates: reproduction
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/j.placenta.2012.11.015

Abstract Studying proteins expressed in placenta is important to understand its function in pregnancy and fetal growth. Here, we present protein expression profiling from normal human placenta by 2-D gel – MS/MS approach that resulted in identification of 117 unique proteins. Integration with earlier analyses resulted in a profile of 423 non-redundant proteins, 75 of them being new identifications unique to this study including their isoforms. We present a compilation of placental protein expressions identified by proteomic approaches, their functions and known clinical implications. We believe that our dataset would be a useful resource for studies related to placental dysfunction.