The Mechanism of Specific Cleavage of Supercoiled DNA by Human DNA Topoisomerase I: The Effect of Ligand Structure on the Catalytic Step of Cleavage

Eukaryotic DNA topoisomerase I (Topo) regulates the topological state of cell DNA and plays an important part in replication, transcription, repair, and recombination. Factors affecting the specific recognition of topologically stressed DNA were analyzed on the basis of the thermodynamic and kinetic...

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Bibliographic Details
Published in:Molecular biology (New York) 2003-03, Vol.37 (2), p.281-292
Main Authors: Bugreev, D V, Buneva, V N, Nevinsky, G A
Format: Article
Language:English
Subjects:
Data processing
Deoxyribonucleic acid
DNA
DNA topoisomerase
Ionizing radiation
Kinetics
Recombination
Replication
Thermodynamics
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Summary:Eukaryotic DNA topoisomerase I (Topo) regulates the topological state of cell DNA and plays an important part in replication, transcription, repair, and recombination. Factors affecting the specific recognition of topologically stressed DNA were analyzed on the basis of the thermodynamic and kinetic data on the Topo-DNA interaction and the X-ray data on human Topo. A model was advanced for possible structural changes occurring in the ligand after initial recognition. The effect of conformational changes in specific DNA on the catalytic step of the reaction was analyzed.[PUBLICATION ABSTRACT]
ISSN:0026-8933
1608-3245
DOI:10.1023/A:1023306023933