Detailed Analysis of Galactooligosaccharides Synthesis with β‑Galactosidase from Aspergillus oryzae

The synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from Aspergillus oryzae (Enzeco) was studied. Using 400 g/L of lactose and 15 U/mL, maximum GOS yield, measured by HPAEC-PAD, was 26.8% w/w of total carbohydrates, obtained at approximately 70% lactose conversion. No less th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2013-02, Vol.61 (5), p.1081-1087
Main Authors: Urrutia, Paulina, Rodriguez-Colinas, Bárbara, Fernandez-Arrojo, Lucía, Ballesteros, Antonio O, Wilson, Lorena, Illanes, Andrés, Plou, Francisco J
Format: Article
Language:English
Subjects:
Aspergillus oryzae
Aspergillus oryzae - enzymology
Bacillus - enzymology
Bacillus circulans
beta-galactosidase
beta-Galactosidase - metabolism
Biological and medical sciences
Disaccharides - biosynthesis
Food industries
Fundamental and applied biological sciences. Psychology
galactooligosaccharides
Hydrogen-Ion Concentration
Kluyveromyces - enzymology
Kluyveromyces marxianus var. lactis
lactose
Lactose - biosynthesis
Magnetic Resonance Spectroscopy
Temperature
Trisaccharides - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from Aspergillus oryzae (Enzeco) was studied. Using 400 g/L of lactose and 15 U/mL, maximum GOS yield, measured by HPAEC-PAD, was 26.8% w/w of total carbohydrates, obtained at approximately 70% lactose conversion. No less than 17 carbohydrates were identified; the major transgalactosylation product was 6′-O-β-galactosyl-lactose, representing nearly one-third (in weight) of total GOS. In contrast with previous reports, the presence of at least five disaccharides was detected, which accounted for 40% of the total GOS at the point of maximum GOS concentration (allolactose and 6-galactobiose were the major products). A. oryzae β-galactosidase showed a preference to form β(1→6) bonds, followed by β(1→3) and β(1→4) linkages. Results were compared with those obtained with β-galactosidases from Kluyveromyces lactis and Bacillus circulans. The highest GOS yield and specific productivity were achieved with B. circulans β-galactosidase. The specificity of the linkages formed and distribution of di-, tri-, and higher GOS varied significantly among the three β-galactosidases.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf304354u