Identification by Q-PCR of Trypanosoma cruzi lineage and determination of blood meal sources in triatomine gut samples in México

Abstract Triatomine vectors were collected on human dwellings in Michoacán México. Blood meal sources were identified by real time polymerase chain reaction (Q-PCR) using DNA extracted from triatomine guts. The assay was performed with one only specific primer set to amplify a fragment of the mitoch...

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Bibliographic Details
Published in:Parasitology international 2013-02, Vol.62 (1), p.36-43
Main Authors: Ibáñez-Cervantes, Gabriela, Martínez-Ibarra, Alejandro, Nogueda-Torres, Benjamín, López-Orduña, Eduardo, Alonso, Ana L, Perea, Cynthia, Maldonado, Teresa, Hernández, José Manuel, León-Avila, Gloria
Format: Article
Language:English
Subjects:
Animals
Base Sequence
blood meal
Blood meals
Chagas disease
Chagas Disease - parasitology
Chagas Disease - transmission
digestive system
Digestive tract
DNA
DNA primers
DTU
Feeding sources
Gastroenterology and Hepatology
Gastrointestinal Contents - chemistry
Gene amplification
genes
Genotype
Humans
Infection
Infectious Disease
Insect Vectors - parasitology
Mexico
Mice
Microscopy
Mitochondria
Molecular Sequence Data
parasites
parasitology
Polymerase Chain Reaction
Primers
Q-PCR
residential housing
RNA, Ribosomal - genetics
RNA, Ribosomal, 18S - genetics
Sequence Alignment
Species Specificity
Triatoma - parasitology
Triatominae
Trypanosoma cruzi
Trypanosoma cruzi - genetics
Vectors
Vertebrates - genetics
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Summary:Abstract Triatomine vectors were collected on human dwellings in Michoacán México. Blood meal sources were identified by real time polymerase chain reaction (Q-PCR) using DNA extracted from triatomine guts. The assay was performed with one only specific primer set to amplify a fragment of the mitochondrial 12S ribosomal gene from vertebrate species. Also Trypanosoma cruzi parasites were detected in triatomine gut samples by microscopy and the positive infection was tested in mice. In addition T. cruzi discrete taxonomic units (DTUs) were identified by Q-PCR with two sets of primers that amplify the mini-circle region (miniexon) and 18S ribosomal mitochondrial gene. The sequences obtained from 18S ribosomal gene amplifications confirmed the presence of T. cruzi I and II lineages, and provide evidence of the presence of lineage TcIII and TcIV.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2012.09.003