Effect of alkylphospholipids on Candida albicans biofilm formation and maturation

The aim of this study was to evaluate miltefosine and four synthetic compounds (TCAN26, TC19, TC106 and TC117) for their in vitro inhibitory activity against Candida albicans planktonic and biofilm cells and investigate whether these compounds are able to inhibit the biofilm formation and to reduce...

Full description

Saved in:
Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2013-01, Vol.68 (1), p.113-125
Main Authors: Vila, Taissa V M, Ishida, Kelly, de Souza, Wanderley, Prousis, Kyriakos, Calogeropoulou, Theodora, Rozental, Sonia
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Bacteria
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Candida albicans
Candida albicans - drug effects
Candida albicans - growth & development
Cells
Fungi
Lipids
Phospholipids - pharmacology
Phosphorylcholine - analogs & derivatives
Phosphorylcholine - pharmacology
Plankton
Plankton - drug effects
Plankton - microbiology
Scanning electron microscopy
Treatment Outcome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to evaluate miltefosine and four synthetic compounds (TCAN26, TC19, TC106 and TC117) for their in vitro inhibitory activity against Candida albicans planktonic and biofilm cells and investigate whether these compounds are able to inhibit the biofilm formation and to reduce the viability of mature C. albicans biofilm cells. The XTT reduction assay and transmission and scanning electron microscopy were employed to determine the inhibitory effects of the test compounds in comparison with amphotericin B and fluconazole against both planktonic cells and sessile cells in biofilms. C. albicans planktonic cells were susceptible to miltefosine, TCAN26 and TC19, all alkylphospholipid compounds. Miltefosine and TCAN26 present a fungicidal activity with similar values of MIC and minimum fungicidal concentration (MFC), ranging from 2 to 8 mg/L. Cell treatment with sub-inhibitory concentrations of alkylphospholipids induced several ultrastructural alterations. In relation to biofilms, miltefosine reduced formation (38%-71%) and mature biofilms viability (32%-44%), at concentrations of 64 mg/L. TCAN26 also reduced biofilm formation (24%-30%) and mature biofilm viability (15%-20%), at concentrations of 64 mg/L. Although amphotericin B reduced biofilm formation similarly to miltefosine (51%-74%), its activity was lower on mature biofilms (24%-30%). Miltefosine antibiofilm activity was significantly higher than amphotericin B, on both formation and mature biofilms (P
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dks353