Evaluation of apoptosis regulatory proteins in response to PUVA therapy for psoriasis

Summary Background The histopathologic changes characteristic of psoriasis might be related to suppressed apoptosis. One of the actions of psoralen ultraviolet A (PUVA) in psoriasis could be exerted through induction of apoptosis of keratinocytes and lymphocytes; however, its exact molecular mechani...

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Bibliographic Details
Published in:Photodermatology, photoimmunology & photomedicine photoimmunology & photomedicine, 2013-02, Vol.29 (1), p.18-26
Main Authors: El-Domyati, Moetaz, Moftah, Noha H., Nasif, Ghada A., Abdel-Wahab, Hossam M., Barakat, Manal T., Abdel-Aziz, Rasha T.
Format: Article
Language:English
Subjects:
Adult
Apoptosis
Apoptosis - drug effects
Apoptosis - radiation effects
Apoptosis Regulatory Proteins - biosynthesis
Bax protein
Bcl-2
Bcl-2 protein
Biological and medical sciences
Biopsy
CD95 antigen
Dermatology
DNA nucleotidylexotransferase
Fas antigen
Female
Gene Expression Regulation - drug effects
Gene Expression Regulation - radiation effects
Humans
Keratinocytes
Keratinocytes - metabolism
Keratinocytes - pathology
Lymphocytes
Male
Medical sciences
Middle Aged
Molecular modelling
P53
p53 protein
Plaques
psoralen
Psoriasis
Psoriasis - drug therapy
Psoriasis - metabolism
Psoriasis - pathology
Psoriasis. Parapsoriasis. Lichen
PUVA
PUVA Therapy
regulatory proteins
Skin
Skin diseases
Time Factors
U.V. radiation
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Summary:Summary Background The histopathologic changes characteristic of psoriasis might be related to suppressed apoptosis. One of the actions of psoralen ultraviolet A (PUVA) in psoriasis could be exerted through induction of apoptosis of keratinocytes and lymphocytes; however, its exact molecular mechanism is still confusing. Aim In this study, we evaluated the expression of pro‐apoptotic (P53, Fas and Bax) and anti‐apoptotic (Bcl‐2) proteins correlating it with apoptotic index (AI) and epidermal thickness in psoriatic skin before and after PUVA therapy. Methods Lesional and non‐lesional skin biopsy specimens were obtained from 10 patients with generalized plaque psoriasis before and after 8 weeks of PUVA therapy. Histometric measurements of epidermal thickness as well as P53, Fas, Bax and Bcl‐2 expressions were evaluated using immunoperoxidase technique and apoptotic cells were detected by terminal deoxynucleotide transferase (TdT) mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. Results After PUVA therapy, the epidermal thickness of psoriatic skin was significantly decreased (P 
ISSN:0905-4383
1600-0781
DOI:10.1111/phpp.12012