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Evaluation of larvicidal activity of soil microbial isolates (Bacillus and Acinetobactor Sp.) against Aedes aegypti (Diptera: Culicidae) - the vector of Chikungunya and Dengue
To isolate and identify the natural non- harmful microbial population from the soil sample for the control of many epidemiological disease causing vector of Aedes aegypti mosquito larvae via larvicidal activity. Heat treat and non heat treated soil samples were used for the isolation of spore formin...
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Published in: | Proceedings of the International Academy of Ecology and Environmental Sciences 2011-12, Vol.1 (3-4), p.169-178 |
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creator | Radhika, D Ramathilaga, A Prabu, C Sathesh Murugesan, A G |
description | To isolate and identify the natural non- harmful microbial population from the soil sample for the control of many epidemiological disease causing vector of Aedes aegypti mosquito larvae via larvicidal activity. Heat treat and non heat treated soil samples were used for the isolation of spore forming and non- spore forming microbial isolates through spread plate technique. Preliminary and quantitative larvicidal activity was performed against the mosquito larvae. Protein analyses of best microbial isolates were carried out by SDSPAGE technique. For the screening assay ten microbial isolates were used and five were active against chosen mosquito larvae which were identified as B. megaterium, B. sphaericus, B. cereus, B. subtillis and Acinetobactor sp. under laboratory conditions. The two isolates (B. megaterium and Acinetobactor sp.) were considered as most toxic strains followed by B. sphaericus, B. cereus, and B. subtillis with LC90 values 4.1 +-0.39, 2.8 +- 0.17, 3.6 +- 0.37, 2.5 +-0.71, 3.6 +- 0.71 mg/ml respectively under laboratory conditions and ensuring mortality rate was 97% at 48 hrs exposure tests. This study concludes that non spore formers of common microbial isolates from the natural environment were also able to kill the larvae of A.aegypti through their secondary metabolites which are non - toxic to human population. |
doi_str_mv | 10.0000/issn-2220-8860-piaees-2011-v1-0015 |
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Heat treat and non heat treated soil samples were used for the isolation of spore forming and non- spore forming microbial isolates through spread plate technique. Preliminary and quantitative larvicidal activity was performed against the mosquito larvae. Protein analyses of best microbial isolates were carried out by SDSPAGE technique. For the screening assay ten microbial isolates were used and five were active against chosen mosquito larvae which were identified as B. megaterium, B. sphaericus, B. cereus, B. subtillis and Acinetobactor sp. under laboratory conditions. The two isolates (B. megaterium and Acinetobactor sp.) were considered as most toxic strains followed by B. sphaericus, B. cereus, and B. subtillis with LC90 values 4.1 +-0.39, 2.8 +- 0.17, 3.6 +- 0.37, 2.5 +-0.71, 3.6 +- 0.71 mg/ml respectively under laboratory conditions and ensuring mortality rate was 97% at 48 hrs exposure tests. This study concludes that non spore formers of common microbial isolates from the natural environment were also able to kill the larvae of A.aegypti through their secondary metabolites which are non - toxic to human population.</description><identifier>ISSN: 2220-8860</identifier><identifier>EISSN: 2220-8860</identifier><identifier>DOI: 10.0000/issn-2220-8860-piaees-2011-v1-0015</identifier><language>eng</language><publisher>Hong Kong: International Academy of Ecology and Environmental Sciences</publisher><subject>Aedes aegypti ; Bacillus ; Bacillus megaterium ; Bacillus sphaericus ; Bacillus thuringiensis ; Bacteria ; Culicidae ; Dengue fever ; Diptera ; Drinking water ; Drug resistance ; Ecology ; Environmental science ; Indexing in process ; Laboratories ; lethal concentration ; Mosquitoes ; Nematodes ; Toxicity ; Tropical diseases</subject><ispartof>Proceedings of the International Academy of Ecology and Environmental Sciences, 2011-12, Vol.1 (3-4), p.169-178</ispartof><rights>Copyright International Academy of Ecology and Environmental Sciences (IAEES) Dec 2011</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1268743660?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590</link.rule.ids></links><search><creatorcontrib>Radhika, D</creatorcontrib><creatorcontrib>Ramathilaga, A</creatorcontrib><creatorcontrib>Prabu, C Sathesh</creatorcontrib><creatorcontrib>Murugesan, A G</creatorcontrib><title>Evaluation of larvicidal activity of soil microbial isolates (Bacillus and Acinetobactor Sp.) against Aedes aegypti (Diptera: Culicidae) - the vector of Chikungunya and Dengue</title><title>Proceedings of the International Academy of Ecology and Environmental Sciences</title><description>To isolate and identify the natural non- harmful microbial population from the soil sample for the control of many epidemiological disease causing vector of Aedes aegypti mosquito larvae via larvicidal activity. Heat treat and non heat treated soil samples were used for the isolation of spore forming and non- spore forming microbial isolates through spread plate technique. Preliminary and quantitative larvicidal activity was performed against the mosquito larvae. Protein analyses of best microbial isolates were carried out by SDSPAGE technique. For the screening assay ten microbial isolates were used and five were active against chosen mosquito larvae which were identified as B. megaterium, B. sphaericus, B. cereus, B. subtillis and Acinetobactor sp. under laboratory conditions. The two isolates (B. megaterium and Acinetobactor sp.) were considered as most toxic strains followed by B. sphaericus, B. cereus, and B. subtillis with LC90 values 4.1 +-0.39, 2.8 +- 0.17, 3.6 +- 0.37, 2.5 +-0.71, 3.6 +- 0.71 mg/ml respectively under laboratory conditions and ensuring mortality rate was 97% at 48 hrs exposure tests. This study concludes that non spore formers of common microbial isolates from the natural environment were also able to kill the larvae of A.aegypti through their secondary metabolites which are non - toxic to human population.</description><subject>Aedes aegypti</subject><subject>Bacillus</subject><subject>Bacillus megaterium</subject><subject>Bacillus sphaericus</subject><subject>Bacillus thuringiensis</subject><subject>Bacteria</subject><subject>Culicidae</subject><subject>Dengue fever</subject><subject>Diptera</subject><subject>Drinking water</subject><subject>Drug resistance</subject><subject>Ecology</subject><subject>Environmental science</subject><subject>Indexing in process</subject><subject>Laboratories</subject><subject>lethal concentration</subject><subject>Mosquitoes</subject><subject>Nematodes</subject><subject>Toxicity</subject><subject>Tropical diseases</subject><issn>2220-8860</issn><issn>2220-8860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNks1qHDEMx4fSQEOaRygYetkUnNgej9fT23aSfkBIDmnPi3ZG3ih1xtOxZ2Cfqq9Y76aU0FN0kSz99BdGKooPUpyLbBcUY8-VUoJbawQfCBAjV0JKPksuhKxeFcf_6q-fxW-K0xgfsoYUpbZGHxe_r2bwEyQKPQuOeRhnaqkDz6BNNFPa7dMxkGeP1I5hQ7lEMXhIGNniE7Tk_RQZ9B1btdRjCpvcGUZ2N5yfMdgC9TGxFXYZB9zuhkRscUlDwhE-smbyh3F4xjhL98hmPDTnmc09_Zz67dTv4KB-ifmBb4sjBz7i6V9_Uvz4fPW9-cqvb798a1bX3EldS25q1KVztrKuRSdLWSlntOg2aKWpnHK2g0pslmhq6Gqou7rGSoJz5VIpU-rypFg86Q5j-DVhTOtHii16Dz2GKa6lspWoTVXJl6KleQEqlLBayVpk9P1_6EOYxj7_OQsau9SlMXvq3RPlIKxhO1JcNzf7S1B5yVqXfwCzvarj</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Radhika, D</creator><creator>Ramathilaga, A</creator><creator>Prabu, C Sathesh</creator><creator>Murugesan, A G</creator><general>International Academy of Ecology and Environmental Sciences</general><general>International Academy of Ecology and Environmental Sciences (IAEES)</general><scope>FBQ</scope><scope>7ST</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BVBZV</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>SOI</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7U6</scope><scope>7U9</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20111201</creationdate><title>Evaluation of larvicidal activity of soil microbial isolates (Bacillus and Acinetobactor Sp.) against Aedes aegypti (Diptera: Culicidae) - the vector of Chikungunya and Dengue</title><author>Radhika, D ; 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Heat treat and non heat treated soil samples were used for the isolation of spore forming and non- spore forming microbial isolates through spread plate technique. Preliminary and quantitative larvicidal activity was performed against the mosquito larvae. Protein analyses of best microbial isolates were carried out by SDSPAGE technique. For the screening assay ten microbial isolates were used and five were active against chosen mosquito larvae which were identified as B. megaterium, B. sphaericus, B. cereus, B. subtillis and Acinetobactor sp. under laboratory conditions. The two isolates (B. megaterium and Acinetobactor sp.) were considered as most toxic strains followed by B. sphaericus, B. cereus, and B. subtillis with LC90 values 4.1 +-0.39, 2.8 +- 0.17, 3.6 +- 0.37, 2.5 +-0.71, 3.6 +- 0.71 mg/ml respectively under laboratory conditions and ensuring mortality rate was 97% at 48 hrs exposure tests. This study concludes that non spore formers of common microbial isolates from the natural environment were also able to kill the larvae of A.aegypti through their secondary metabolites which are non - toxic to human population.</abstract><cop>Hong Kong</cop><pub>International Academy of Ecology and Environmental Sciences</pub><doi>10.0000/issn-2220-8860-piaees-2011-v1-0015</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Aedes aegypti Bacillus Bacillus megaterium Bacillus sphaericus Bacillus thuringiensis Bacteria Culicidae Dengue fever Diptera Drinking water Drug resistance Ecology Environmental science Indexing in process Laboratories lethal concentration Mosquitoes Nematodes Toxicity Tropical diseases |
title | Evaluation of larvicidal activity of soil microbial isolates (Bacillus and Acinetobactor Sp.) against Aedes aegypti (Diptera: Culicidae) - the vector of Chikungunya and Dengue |
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