Antimicrobial resistance patterns and their encoding genes among Acinetobacter baumannii strains isolated from burned patients

• Published in: Burns 2012-12, Vol.38 (8), p.1198-1203
• Main Authors: Asadollahi, Parisa, Akbari, Mahdi, Soroush, Setareh, Taherikalani, Morovat, Asadollahi, Khairollah, Sayehmiri, Kourosh, Maleki, Abbas, Maleki, Mohammad Hossein, Karimi, Parviz, Emaneini, Mohammad
• Format: Article
• Language: English
• Subjects: A. baumannii; Acinetobacter baumannii; Acinetobacter baumannii - drug effects; Acinetobacter baumannii - genetics; Acinetobacter baumannii - isolation & purification; Acinetobacter Infections - drug therapy; Acinetobacter Infections - microbiology; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Biological and medical sciences; Burns; Burns - microbiology; Critical Care; Drug Resistance, Bacterial - genetics; Humans; Iran; Medical sciences; Microbial Sensitivity Tests; Resistant genes; Traumas. Diseases due to physical agents
• ISSN: 0305-4179; 1879-1409
• DOI: 10.1016/j.burns.2012.04.008

Abstract The purpose of this study was to determine the mechanisms and patterns of antimicrobial resistance among the isolates obtained from burned patients with wound infections at a teaching hospital in Tehran, Iran. A total of 23 Acinetobacter baumannii isolates were collected from patients with burn wound infections between August 2009 and July 2010 from a hospital in Tehran. The susceptibility of these strains against 11 antimicrobial agents was determined by E-test according to the CLSI guidelines. All the resistant strains were then subjected to PCR assay for 28 distinct resistance genes. The most active antimicrobial agent was colistin with 100% sensitivity followed by gentamicin, amikacin and imipenem with 69.5%, 52.1% and 51.1% sensitivity, respectively. The most frequent resistance genes detected were bla OXA-51-like genes ( n = 23; 100%) that was intrinsic to A. baumannii isolates, gyrA ( n = 23; 100%), carO ( n = 23; 100%), tetA ( n = 22; 95.5%), tetB ( n = 15; 65.2%), intI ( n = 13; 56.5%) and PER ( n = 12; 52.1%), respectively. In order to make a proper choice of antibiotic for burn patients, it would be beneficial to physicians to identify drug resistance patterns in A. baumannii isolates.