Photodynamic Inactivation of Planktonic Cultures and Biofilms of Candida albicans Mediated by Aluminum-Chloride-Phthalocyanine Entrapped in Nanoemulsions

• Published in: Photochemistry and photobiology 2013-01, Vol.89 (1), p.111-119
• Main Authors: Ribeiro, Ana Paula Dias, Andrade, Mariana Carvalho, de Fátima da Silva, Julhiany, Jorge, Janaina Habib, Primo, Fernando Lucas, Tedesco, Antonio Cláudio, Pavarina, Ana Cláudia
• Format: Article
• Language: English
• Subjects: Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Biofilms - radiation effects; Candida albicans; Candida albicans - drug effects; Candida albicans - growth & development; Candida albicans - radiation effects; Cell Membrane - drug effects; Cell Membrane - radiation effects; Cells; Colony Count, Microbial; Drug Carriers - chemistry; Emulsions; Indoles - chemistry; Indoles - pharmacology; Light; Light emitting diodes; Metabolism; Microbial Sensitivity Tests; Microbial Viability - drug effects; Microbial Viability - radiation effects; Nanospheres - chemistry; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacology; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacology; Plankton; Plankton - drug effects; Plankton - growth & development; Plankton - radiation effects; Static Electricity
• ISSN: 0031-8655; 1751-1097
• DOI: 10.1111/j.1751-1097.2012.01198.x

New drug delivery systems, such as nanoemulsions (NE), have been developed to allow the use of hydrophobic drugs on the antimicrobial photodynamic therapy. This study evaluated the photodynamic potential of aluminum‐chloride‐phthalocyanine (ClAlPc) entrapped in cationic and anionic NE to inactivate Candida albicans planktonic cultures and biofilm compared with free ClAlPc. Fungal suspensions were treated with different delivery systems containing ClAlPc and light emitting diode. For planktonic suspensions, colonies were counted and cell metabolism was evaluated by XTT assay. Flow cytometry evaluated cell membrane damage. For biofilms, the metabolic activity was evaluated by XTT and ClAlPc distribution through biofilms was analyzed by confocal laser scanning microscopy (CLSM). Fungal viability was dependent on the delivery system, superficial charge and light dose. Free ClAlPc caused photokilling of the yeast when combined with 100 J cm−2. Cationic NE‐ClAlPc reduced significantly both colony counts and cell metabolism (P