BDNF expression with functional improvement in transected spinal cord treated with neural stem cells in adult rats

Abstract Neural stem cells (NSC) could promote the repair after spinal cord transection (SCT), the underlying mechanism, however, still keeps to be defined. This study reported that NSC grafts significantly improved sensory and locomotor functions in adult rats with SCT in acute stage after injury....

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Bibliographic Details
Published in:Neuropeptides (Edinburgh) 2013-02, Vol.47 (1), p.1-7
Main Authors: He, Bao-Li, Ba, Ying-chun, Wang, Xu-yang, Liu, Su-juan, Liu, Guo-dong, Ou, Shan, Gu, Yin-li, Pan, Xing-hua, Wang, Ting-Hua
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
BDNF
Biotin
Biotin - analogs & derivatives
Brain-Derived Neurotrophic Factor - biosynthesis
Brain-Derived Neurotrophic Factor - genetics
Cell Differentiation - physiology
Cell Survival - physiology
Dextrans
Endocrinology & Metabolism
Evoked Potentials, Somatosensory - drug effects
Evoked Potentials, Somatosensory - physiology
Fluorescent Antibody Technique
Fluorescent Dyes
Hindlimb - innervation
Hindlimb - physiology
Immunohistochemistry
Locomotion - physiology
Motor Activity - drug effects
Neural Stem Cells - transplantation
NSC
Rat
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Recovery of Function
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Sensory and locomotor functions
Spinal Cord - metabolism
Spinal Cord Injuries - metabolism
Spinal Cord Injuries - therapy
Transplantation
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Summary:Abstract Neural stem cells (NSC) could promote the repair after spinal cord transection (SCT), the underlying mechanism, however, still keeps to be defined. This study reported that NSC grafts significantly improved sensory and locomotor functions in adult rats with SCT in acute stage after injury. NSC could survive; differentiate towards neurons or glia lineage in vitro and vivo. Biotin dextran amine (BDA) tracing showed that little CST regeneration in the injury site, while SEP was recorded in NSC engrafted rats. Immunohistochemistry and Real time PCR confirmed that engrafted NSC expressed BDNF and increased the level of BDNF mRNA in injured site following transplantation. The present data therefore suggested that the functional recovery following SCT with NSC transplantation was correlated with the expression of BDNF, indicating the usage of BDNF with NSC transplantation in the treatment of SCI following injury.
ISSN:0143-4179
1532-2785
DOI:10.1016/j.npep.2012.06.001