The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis

Smoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF–MS analysis, we found that the protein profile of the mouse e...

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Bibliographic Details
Published in:The international journal of biochemistry & cell biology 2013-03, Vol.45 (3), p.571-582
Main Authors: Zhu, Zijue, Xu, Wangjie, Dai, Jingbo, Chen, Xiaohui, Zhao, Xianglong, Fang, Peng, Yang, Fang, Tang, Maoping, Wang, Zhaoxia, Wang, Lianyun, Ma, Duan, Qaio, Zhongdong
Format: Article
Language:English
Subjects:
2-D Gel electrophoresis
Animals
Cigarette smoking
cigarettes
Electrophoresis, Gel, Two-Dimensional
endoplasmic reticulum
energy metabolism
environmental exposure
Epididymis
Epididymis - metabolism
gels
gene expression regulation
glutathione
Humans
immunohistochemistry
Male
Male fertility
Mass spectrometry
matrix-assisted laser desorption-ionization mass spectrometry
Mice
oxidative stress
proteins
Proteins - metabolism
reproduction
semen
Semen Analysis
smoke
Smoking
smoking (habit)
Transcriptome - genetics
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Summary:Smoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF–MS analysis, we found that the protein profile of the mouse epididymis was altered by cigarette smoking and identified 27 proteins from the most abundant and differentially expressed spots in the 2-DE gels of epididymal samples. These proteins were classified into groups according to their functions such as energy metabolism, reproduction and structural molecule activity. Through pathway analysis, these proteins were associated with the glutathione metabolism and protein processing in the endoplasmic reticulum. These results showed that the epididymis may experience oxidative stress following cigarette smoke exposure, which was confirmed using immunohistochemistry. We determine that cigarette smoking can induce oxidative stress in the mouse epididymis, which may cause protein profile altering, thereby impairing epididymis function, and leading to a decline in semen quality.
ISSN:1357-2725
1878-5875
DOI:10.1016/j.biocel.2012.12.007