Quercetin in elimination of tumor initiating stem-like and mesenchymal transformation property in head and neck cancer

Background Previously, we enriched a subpopulation of head and neck cancer–derived tumor initiating cells (HNC‐TICs) presented high tumorigenic, chemo‐radioresistant, and coupled with epithelial–mesenchymal transition (EMT) properties. The purpose of this study was to investigate the therapeutic eff...

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Published in:Head & neck 2013-03, Vol.35 (3), p.413-419
Main Authors: Chang, Wen-Wei, Hu, Fang-Wei, Yu, Cheng-Chia, Wang, Hsiu-Huan, Feng, Hsiang-Pu, Lan, Chih, Tsai, Lo-Lin, Chang, Yu-Chao
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Line, Tumor
Cell Transformation, Neoplastic - drug effects
epithelial-mesenchymal transition
Epithelial-Mesenchymal Transition - drug effects
Gene Expression Regulation, Neoplastic
head and neck cancer
Head and Neck Neoplasms - drug therapy
Humans
Isoenzymes - antagonists & inhibitors
metastasis
Neoplastic Stem Cells - drug effects
quercetin
Quercetin - therapeutic use
Retinal Dehydrogenase - antagonists & inhibitors
tumor initiating cells
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Summary:Background Previously, we enriched a subpopulation of head and neck cancer–derived tumor initiating cells (HNC‐TICs) presented high tumorigenic, chemo‐radioresistant, and coupled with epithelial–mesenchymal transition (EMT) properties. The purpose of this study was to investigate the therapeutic effect and molecular mechanisms of quercetin on HNC‐TICs. Method ALDH1 activity of head and neck cancer cells with quercetin treatment was assessed by the Aldefluor assay flow cytometry analysis. Self‐renewal, invasiveness, and EMT capability of HNC‐TICs with different doses of quercetin was presented. Results We first observed that the treatment of quercetin significantly downregulated the ALDH1 activity of head and neck cancer cells in a dose‐dependent manner (p < .05). Moreover, quercetin reduced self‐renewal property and stemness signatures expression in head and neck cancer‐derived sphere cells. The migration ability of head and neck cancer‐derived sphere cells was lessened under quercetin treatment partially due to the decreased productions of Twist, N‐cadherin, and vimentin. Conclusion Quercetin suppressing HNC‐TICs characteristics may therefore be valuable therapeutics clinically in combination with standard treatment modalities. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
ISSN:1043-3074
1097-0347
DOI:10.1002/hed.22982