An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the V max and K m was 370.17 U/ml/min and 6.18 mg/ml re...

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Bibliographic Details
Published in:Carbohydrate polymers 2011-01, Vol.83 (2), p.891-897
Main Authors: Trivedi, Nitin, Gupta, Vishal, Kumar, Manoj, Kumari, Puja, C.R.K.Reddy, Jha, B.
Format: Article
Language:English
Subjects:
Bacillus
Biological and medical sciences
Biotechnology
Cellulase
Cellulose
Fundamental and applied biological sciences. Psychology
Halo-alkali tolerance
Marine habitat
Mission oriented research
Prospectives
Ulva lactuca
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Summary:An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the V max and K m was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0–12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li 1+ were found as inducers while Cr 2+, Co 2+, Zn 2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2010.08.069