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An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca
An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the V max and K m was 370.17 U/ml/min and 6.18 mg/ml re...
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| Published in: | Carbohydrate polymers 2011-01, Vol.83 (2), p.891-897 |
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| Main Authors: | , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c372t-2da49b6093f849d0483fec09e2ae29a0ccecd45ac536141986e03265afc782b23 |
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| cites | cdi_FETCH-LOGICAL-c372t-2da49b6093f849d0483fec09e2ae29a0ccecd45ac536141986e03265afc782b23 |
| container_end_page | 897 |
| container_issue | 2 |
| container_start_page | 891 |
| container_title | Carbohydrate polymers |
| container_volume | 83 |
| creator | Trivedi, Nitin Gupta, Vishal Kumar, Manoj Kumari, Puja C.R.K.Reddy Jha, B. |
| description | An extracellular alkali-halotolerant cellulase from the strain
Bacillus flexus NT isolated from
Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97
kDa and the
V
max and
K
m was 370.17
U/ml/min and 6.18
mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45
°C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0–12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd
2+ and Li
1+ were found as inducers while Cr
2+, Co
2+, Zn
2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene. |
| doi_str_mv | 10.1016/j.carbpol.2010.08.069 |
| format | article |
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Bacillus flexus NT isolated from
Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97
kDa and the
V
max and
K
m was 370.17
U/ml/min and 6.18
mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45
°C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0–12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd
2+ and Li
1+ were found as inducers while Cr
2+, Co
2+, Zn
2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2010.08.069</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Bacillus ; Biological and medical sciences ; Biotechnology ; Cellulase ; Cellulose ; Fundamental and applied biological sciences. Psychology ; Halo-alkali tolerance ; Marine habitat ; Mission oriented research ; Prospectives ; Ulva lactuca</subject><ispartof>Carbohydrate polymers, 2011-01, Vol.83 (2), p.891-897</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-2da49b6093f849d0483fec09e2ae29a0ccecd45ac536141986e03265afc782b23</citedby><cites>FETCH-LOGICAL-c372t-2da49b6093f849d0483fec09e2ae29a0ccecd45ac536141986e03265afc782b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23473942$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Trivedi, Nitin</creatorcontrib><creatorcontrib>Gupta, Vishal</creatorcontrib><creatorcontrib>Kumar, Manoj</creatorcontrib><creatorcontrib>Kumari, Puja</creatorcontrib><creatorcontrib>C.R.K.Reddy</creatorcontrib><creatorcontrib>Jha, B.</creatorcontrib><title>An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca</title><title>Carbohydrate polymers</title><description>An extracellular alkali-halotolerant cellulase from the strain
Bacillus flexus NT isolated from
Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97
kDa and the
V
max and
K
m was 370.17
U/ml/min and 6.18
mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45
°C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0–12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd
2+ and Li
1+ were found as inducers while Cr
2+, Co
2+, Zn
2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.</description><subject>Bacillus</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cellulase</subject><subject>Cellulose</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Halo-alkali tolerance</subject><subject>Marine habitat</subject><subject>Mission oriented research</subject><subject>Prospectives</subject><subject>Ulva lactuca</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkE1PGzEQhi1UpKbAT0DaCxKXTf0V7_qEUkShUqReyBVrMjtLnTrrYG8C_fd1lKjXzmWkd975ehi7FnwquDBf11OEtNrGMJW8aLydcmPP2ES0ja2F0voTm3Chdd0a0XxmX3Je8xJG8Al7mQ8VhN8QfP0LQhxjoATDWCGFsAuQqepT3FTfAH0RctUH-ijJ5xhgpO5YfU1EQ5UJ3qlIy7CHKgCOO4RLdt5DyHR1yhds-f3h-f6pXvx8_HE_X9SoGjnWsgNtV4Zb1bfadly3qifkliSQtMARCTs9A5wpI7SwrSGupJlBj00rV1JdsNvj3G2KbzvKo9v4fPgBBoq77IS0wnAlbFOss6MVU8w5Ue-2yW8g_XGCuwNPt3Ynnu7A0_HWFZ6l7-a0AjJC6Asm9Plfs1S6UVYfTrk7-qj8u_eUXEZPA1LnE-Houuj_s-kv_daPCQ</recordid><startdate>20110110</startdate><enddate>20110110</enddate><creator>Trivedi, Nitin</creator><creator>Gupta, Vishal</creator><creator>Kumar, Manoj</creator><creator>Kumari, Puja</creator><creator>C.R.K.Reddy</creator><creator>Jha, B.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20110110</creationdate><title>An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca</title><author>Trivedi, Nitin ; Gupta, Vishal ; Kumar, Manoj ; Kumari, Puja ; C.R.K.Reddy ; Jha, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-2da49b6093f849d0483fec09e2ae29a0ccecd45ac536141986e03265afc782b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacillus</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cellulase</topic><topic>Cellulose</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Halo-alkali tolerance</topic><topic>Marine habitat</topic><topic>Mission oriented research</topic><topic>Prospectives</topic><topic>Ulva lactuca</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trivedi, Nitin</creatorcontrib><creatorcontrib>Gupta, Vishal</creatorcontrib><creatorcontrib>Kumar, Manoj</creatorcontrib><creatorcontrib>Kumari, Puja</creatorcontrib><creatorcontrib>C.R.K.Reddy</creatorcontrib><creatorcontrib>Jha, B.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trivedi, Nitin</au><au>Gupta, Vishal</au><au>Kumar, Manoj</au><au>Kumari, Puja</au><au>C.R.K.Reddy</au><au>Jha, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca</atitle><jtitle>Carbohydrate polymers</jtitle><date>2011-01-10</date><risdate>2011</risdate><volume>83</volume><issue>2</issue><spage>891</spage><epage>897</epage><pages>891-897</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>An extracellular alkali-halotolerant cellulase from the strain
Bacillus flexus NT isolated from
Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97
kDa and the
V
max and
K
m was 370.17
U/ml/min and 6.18
mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45
°C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0–12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd
2+ and Li
1+ were found as inducers while Cr
2+, Co
2+, Zn
2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2010.08.069</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0144-8617 |
| ispartof | Carbohydrate polymers, 2011-01, Vol.83 (2), p.891-897 |
| issn | 0144-8617 1879-1344 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Bacillus Biological and medical sciences Biotechnology Cellulase Cellulose Fundamental and applied biological sciences. Psychology Halo-alkali tolerance Marine habitat Mission oriented research Prospectives Ulva lactuca |
| title | An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca |
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