Increased chondrocyte seeding density has no positive effect on cartilage repair in an MPEG-PLGA scaffold

Purpose This study investigates the effect of cell seeding density on cartilage repair in matrix-assisted chondrocyte implantation in vitro and in vivo. Methods In vitro: Four different cell seeding densities of human chondrocytes were seeded onto a porous methoxy-polyethylene glycol-polylactic-co-g...

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Bibliographic Details
Published in:Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA sports traumatology, arthroscopy : official journal of the ESSKA, 2013-02, Vol.21 (2), p.485-493
Main Authors: Hansen, Ole Møller, Foldager, Casper Bindzus, Christensen, Bjørn Borsøe, Everland, Hanne, Lind, Martin
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Biocompatible Materials
Biopsy
Cartilage
Cartilage, Articular - injuries
Cartilage, Articular - physiology
Cell Count
Cell culture
Cell Culture Techniques
Cell Transplantation
Chondrocytes - transplantation
Collagen
Experimental Study
Gene expression
Gene Expression Profiling
Humans
Knee
Knee Joint - physiology
Medicine
Medicine & Public Health
Orthopedics
Polyesters
Polyethylene Glycols
Rabbits
RNA polymerase
Tissue Scaffolds
Transplantation, Autologous
Transplants & implants
Wound Healing - physiology
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Summary:Purpose This study investigates the effect of cell seeding density on cartilage repair in matrix-assisted chondrocyte implantation in vitro and in vivo. Methods In vitro: Four different cell seeding densities of human chondrocytes were seeded onto a porous methoxy-polyethylene glycol-polylactic-co-glycolic acid scaffold (MPEG-PLGA) polymer scaffold ASEED™ (1.2 × 10 6 , 4.0 × 10 6 , 1.2 × 10 7 and 2.0 × 10 7  cells/cm 3 ). The cartilage repair response was evaluated by relative gene expression of the chondrogenic markers sox9, collagen types I, II and X, and aggrecan, total DNA content and sulphated glycosaminoglycan synthesis. In vivo: Using a New Zealand white rabbit intercondylar osteochondral defect model, three different cell seeding densities (1.2 × 10 6 , 4.0 × 10 6 and 1.2 × 10 7  cells/cm 3 ) were tested with an empty scaffold as control. The cartilage repair response was evaluated using O’Driscoll score. Results In vitro: A significant difference ( p  
ISSN:0942-2056
1433-7347
DOI:10.1007/s00167-012-1996-4