Proteome-Based Profiling of Hypercellulase-Producing Strains Developed Through Interspecific Protoplast Fusion Between Aspergillus nidulans and Aspergillus tubingensis

Thirty heterokaryons, formed by protoplast fusion of Aspergillus nidulans and Aspergillus tubingensis , were selected on the basis of their ability to grow on 2-deoxyglucose (0.2 %, w / v ) and intermediate spore color. These heterokaryons were studied for cellulase production using shake flask and...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2013, Vol.169 (2), p.393-407
Main Authors: Kaur, Baljit, Sharma, Manju, Soni, Rohit, Oberoi, H. S., Chadha, B. S.
Format: Article
Language:English
Subjects:
Aspergillus - classification
Aspergillus - growth & development
Aspergillus nidulans
Aspergillus tubingensis
Biochemistry
Biological and medical sciences
Biomass
Biotechnology
Cell Fusion - methods
Cellulase
Cellulase - genetics
Chemistry
Chemistry and Materials Science
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungi
Liquid chromatography
Mass spectroscopy
Oryza sativa
Proteins
Proteome - genetics
Proteomics
Protoplasts - physiology
Rice straw
Species Specificity
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Summary:Thirty heterokaryons, formed by protoplast fusion of Aspergillus nidulans and Aspergillus tubingensis , were selected on the basis of their ability to grow on 2-deoxyglucose (0.2 %, w / v ) and intermediate spore color. These heterokaryons were studied for cellulase production using shake flask and solid substrate cultures at 40 °C. Fusants 51 and 28 exhibited appreciably higher levels of endoglucanase, cellobiohydrolase, β-glucosidase, and FPase activities when compared with parental strains. Employing proteomic-based approaches, the differential expression of proteins in secretome of fusants and parental strains were analyzed using two-dimensional electrophoresis. The expression of some of the proteins in the fusants was found to be up/downregulated. The upregulated proteins in the fusant 51 were identified by liquid chromatography–mass spectroscopy as endoxylanase, endochitinase, β-glucosidase, as well as hypothetical proteins. The cellulases produced by fusants 28 and 51 showed improved saccharification of alkali treated rice straw when compared with the parental strains.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-012-9985-0