Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis

Glutathione (GSH) has been shown to negatively regulate methyl jasmonate (MeJA)-induced stomatal closure. We investigated the roles of GSH in MeJA signaling in guard cells using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase. MeJA...

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Bibliographic Details
Published in:Journal of plant growth regulation 2013-03, Vol.32 (1), p.208-215
Main Authors: Akter, Nasima, Okuma, Eiji, Sobahan, Muhammad Abdus, Uraji, Misugi, Munemasa, Shintaro, Nakamura, Yoshimasa, Mori, Izumi C, Murata, Yoshiyuki
Format: Article
Language:English
Subjects:
Abscisic acid
Agriculture
Arabidopsis
Biomedical and Life Sciences
Biosynthesis
Enzymes
Esters
Glutamate-cysteine ligase
Glutathione
Guard cells
Hydrogen peroxide
Life Sciences
Methyl jasmonate
mutants
Mutation
Plant Anatomy/Development
Plant Physiology
Plant Sciences
Reactive oxygen species
Signal transduction
Stomata
stomatal movement
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Summary:Glutathione (GSH) has been shown to negatively regulate methyl jasmonate (MeJA)-induced stomatal closure. We investigated the roles of GSH in MeJA signaling in guard cells using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase. MeJA-induced stomatal closure and decreased GSH contents in guard cells. Decreasing GSH by the cad2-1 mutation enhanced MeJA-induced stomatal closure. Depletion of GSH by the cad2-1 mutation or increment of GSH by GSH monoethyl ester did not affect either MeJA-induced production of reactive oxygen species (ROS) or MeJA-induced cytosolic alkalization in guard cells. MeJA and abscisic acid (ABA) induced stomatal closure and GSH depletion in atrbohD and atrbohF single mutants but not in the atrbohD atrbohF double mutant. Moreover, exogenous hydrogen peroxide induced stomatal closure but did not deplete GSH in guard cells. These results indicate that GSH affects MeJA signaling as well as ABA signaling and that GSH negatively regulates a signal component other than ROS production and cytosolic alkalization in MeJA signal pathway of Arabidopsis guard cells.
ISSN:0721-7595
1435-8107
DOI:10.1007/s00344-012-9291-7