Effect of Wuziyanzong Pill on sperm quality and calcium ion content in oligoasthenospermia rats

• Published in: Journal of traditional Chinese medicine 2012-12, Vol.32 (4), p.631-635
• Main Authors: Wang, Tongsheng, Huang, Jinling, Wu, Deling, Li, Qing, Liu, Xiangguo, Chen, Han, Qiao, Lei, Li, Dongdong, Li, Jun
• Format: Article
• Language: English
• Subjects: Animals; Calcium - metabolism; Cell Survival - drug effects; Drugs, Chinese Herbal - administration & dosage; Humans; Infertility, Male - drug therapy; Infertility, Male - physiopathology; Male; Oligospermia - drug therapy; Oligospermia - physiopathology; Rats; Sperm Count; Sperm Motility - drug effects; Spermatozoa - abnormalities; Spermatozoa - drug effects; Spermatozoa - metabolism; 原子吸收光谱法; 大鼠模型; 治疗效果; 精子密度; 精子活力; 精子质量; 钙离子含量; 雷公藤多甙
• ISSN: 0255-2922
• DOI: 10.1016/S0254-6272(13)60083-7

OBJECTIVE： To study the effect of Wuziyanzong treatment on the sperm quality and content of cal- cium ions （Ca2＋） in oligoasthenospermia rats. METHODS： A model of oligoasthenospermia was induced in 50 Sprague Dawley rats by treatment with tripterygium glycosides at 30 mg/kg per day for 8 weeks. They were divided randomly into a model group, a positive group （Huangjingzanyu capsule, 3.01 g/kg）, and low, medium and high dose Wuziyanzong treatment groups （2.30, 4.60, 9.20 g/kg crude drug respectively） with 10 in each group. Another 10 rats were used as a control group. The rats in the control and model groups were administered distilled water, while the rats in the remaining groups were administered Wuziyan- zong for 30 d. The epididymides were removed, spermatozoa recovered and the sperm density and viability were measured. The spermatozoa were pu- rified and the contents of Ca2~ in the cytoplasm and mitochondria were detected by flow cytometry and atomic absorption spectrometry, respectively. RESULTS： After 8 weeks of treatment with tripteryg- ium glycosides, the sperm density, sperm activity and the Ca2~ content of spermatozoa in the model rats were all significantly decreased compared with the control group （all P〈O.05）. After 30 d treatment, the sperm density and activity improved and the Ca2＋ content of sperm were increased significantly in the medium and high dose Wuziyanzong treat- ment groups in comparison with the model group （all P〈O.05）. CONCLUSION： The Wuziyanzong treatment in： creased sperm density, improved sperm viability and enhanced the content of Ca2＋ in the sperm cy- toplasm and mitochondria in this rat model of oli- goasthenospermia.