Effect of Lichong Decoction on expression of IGF-I and proliferating cell nuclear antigen mRNA in rat model of uterine leiomyoma

• Published in: Journal of traditional Chinese medicine 2012-12, Vol.32 (4), p.636-640
• Main Authors: Li, Donghua, Zhang, Yalan, Han, Hongjuan, Geng, Jianguo, Xie, Xiaolei, Zheng, Jiubo, Wang, Yasong, Zou, Xiaoli
• Format: Article
• Language: English
• Subjects: Animals; Disease Models, Animal; Drugs, Chinese Herbal - administration & dosage; Female; Gene Expression - drug effects; Humans; IGF-I; Insulin-Like Growth Factor I - genetics; Insulin-Like Growth Factor I - metabolism; Leiomyoma - drug therapy; Leiomyoma - genetics; Leiomyoma - metabolism; Proliferating Cell Nuclear Antigen - genetics; Proliferating Cell Nuclear Antigen - metabolism; Rats; Rats, Wistar; Wistar大鼠; 增殖细胞核抗原; 大鼠模型; 子宫肌瘤; 抗原基因; 荧光定量PCR检测; 透射电子显微镜
• ISSN: 0255-2922
• DOI: 10.1016/S0254-6272(13)60084-9

OBJECTIVE： To study the effect of Lichong Decoc- tion （Lichong Decoction for strengthening an- ti-pathogenic Qi and eliminating blood stasis） on the expression of insulin-like growth factor-I （IGF-I） and proliferating cell nuclear antigen （PCNA） mRNA in a rat model of uterine leiomyoma. METHODS： Fifty female Wistar rats were random- ized into a normal control group, model group, Li- chong Decoction group, Guizhifuling Capsule （Cap- sule containing Cassia Twig and Poria） group, and Mifepristone group. The uterine leiomyoma model was established by peritoneal injections of exoge- nous estrogen and progesterone hormone. The ul- trastructural changes in cells of rat uterine tissues were observed with transmission electron micros- copy, and the expression of IGF-I and PCNA mRNAwas detected by real-time fluorescent quantitative PCR. RESULTS： Following treatment, cells in the Lichong Decoction group appeared to be arranged normal- ly, the cellular morphology were almost in a normal state, hyperplasia and hypertrophy of the chondrio- some was reduced, collagen fibers were arranged in a regular manner, without obvious hyperplasia, and the expression of IGF-I and PCNA mRNA was significantly decreased compared with the model group （P〈0.01）. CONCLUSIONS： The effect of Lichong Decoction on uterine leiomyoma is related to its function in re- ducing the expression of IGF-I and PCNA mRNA.