Arcobacter cloacae sp. nov. and Arcobacter suis sp. nov., two new species isolated from food and sewage

Three strains recovered from mussels (F26), sewage (SW28-13T) and pork meat (F41T) were characterized as Arcobacter. They did not appear to resemble any known species on the basis of their 16S rDNA-RFLP patterns and the rpoB gene analyses. However, strains F26 and SW28-13T appeared to be the same sp...

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Bibliographic Details
Published in:Systematic and applied microbiology 2013-02, Vol.36 (1), p.22-27
Main Authors: Levican, Arturo, Collado, Luis, Figueras, María José
Format: Article
Language:English
Subjects:
Arcobacter
Arcobacter - chemistry
Arcobacter - classification
Arcobacter - genetics
Arcobacter - isolation & purification
Bacterial Typing Techniques
Cluster Analysis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
DNA-Directed RNA Polymerases - genetics
Food
Food Microbiology
gyrA-atpA-rpoB-gyrB-hsp60
MALDI-TOF
Meat - microbiology
MLPA
Molecular Sequence Data
Multilocus Sequence Typing
Nucleic Acid Hybridization
Phylogeny
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Seafood - microbiology
Sewage
Sewage - microbiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Three strains recovered from mussels (F26), sewage (SW28-13T) and pork meat (F41T) were characterized as Arcobacter. They did not appear to resemble any known species on the basis of their 16S rDNA-RFLP patterns and the rpoB gene analyses. However, strains F26 and SW28-13T appeared to be the same species. The 16S rRNA gene sequence similarity of strains SW28-13T and F41T to the type strains of all other Arcobacter species ranged from 94.1% to 99.6% and 93.4% to 98.8%, respectively. Phenotypic characteristics and the DNA–DNA hybridization (DDH) results showed that they belonged to 2 new Arcobacter species. A multilocus phylogenetic analysis (MLPA) with the concatenated sequences of 5 housekeeping genes (gyrA, atpA, rpoB, gyrB and hsp60) was used for the first time in the genus, showing concordance with the 16S rRNA gene phylogenetic analysis and DDH results. The MALDI-TOF mass spectra also discriminated these strains as two new species. The names proposed for them are Arcobacter cloacae with the type strain SW28-13T (=CECT 7834T=LMG 26153T) and Arcobacter suis with the type strain F41T (=CECT 7833T=LMG 26152T).
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2012.11.003