Beneficial effect of splenic artery ligation on bacterial translocation after major liver resection in rats

Abstract Objective In major liver resection, bacterial translocation appears to be an important mechanism in the pathogenesis of spontaneous infection. This study was designed to investigate the effects of splenic artery ligation on bacterial translocation after major liver resection. Materials and...

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Bibliographic Details
Published in:Digestive and liver disease 2013-03, Vol.45 (3), p.233-237
Main Authors: Chen, Wen-Zhe, Hu, Kun-Peng, Xu, Rui-Yun, Pan, Wei-Dong
Format: Article
Language:English
Subjects:
Animals
Bacterial Infections - prevention & control
Bacterial translocation
Bacterial Translocation - physiology
Endotoxin
Endotoxins - blood
Female
Gastroenterology and Hepatology
Gastrointestinal Tract - metabolism
Gastrointestinal Tract - microbiology
Gut barrier
Hepatectomy - methods
Ileum - pathology
Ileum - ultrastructure
Lactic Acid - metabolism
Ligation
Lymph Nodes - microbiology
Major hepatectomy
Permeability
Portal Pressure
Rats
Rats, Sprague-Dawley
Splenic Artery - surgery
Splenic artery ligation
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Summary:Abstract Objective In major liver resection, bacterial translocation appears to be an important mechanism in the pathogenesis of spontaneous infection. This study was designed to investigate the effects of splenic artery ligation on bacterial translocation after major liver resection. Materials and methods Rats were divided into three groups: the sham operation group (SO group), the two-thirds partial hepatectomy group (PHx group) and the two-thirds partial hepatectomy plus splenic artery ligation group (PHx + Sp group). Bacterial translocation, endotoxemia, d -lactic acid and intestinal histology were analyzed among three groups. Results The rate of bacterial translocation was higher in the PHx rats than in the SO rats (65.0% vs. 6.67%; P = 0.001), so that in the PHx + Sp rats (25.0%; P = 0.011). Endotoxemia was not evident in the SO rats (0 pg/ml) and blood endotoxin levels decreased in the PHx + Sp rats (1.47 pg/ml) compared with the PHx rats (4.05 pg/ml, P < 0.001). d -lactic acid was also higher in both the PHx and PHx + Sp rats compared with the SO rats (39.09 mg/ml, 23.36 mg/ml, and 1.68 mg/ml; P < 0.01). Conclusion Splenic artery ligation enhanced intestinal barrier function and diminished blood endotoxin levels and bacterial translocation in rats with major liver resection.
ISSN:1590-8658
1878-3562
DOI:10.1016/j.dld.2012.09.009