Understanding the cellulolytic system of Trichoderma harzianum P49P11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-l-arabinofuranosidase

• Published in: Bioresource technology 2013-03, Vol.131, p.500-507
• Main Authors: Delabona, Priscila da Silva, Cota, Júnio, Hoffmam, Zaira Bruna, Paixão, Douglas Antonio Alvaredo, Farinas, Cristiane Sanchez, Cairo, João Paulo Lourenço Franco, Lima, Deise Juliana, Squina, Fábio Marcio, Ruller, Roberto, Pradella, José Geraldo da Cruz
• Format: Article
• Language: English
• Subjects: Accessory enzymes; Biological and medical sciences; Biotechnology; Carbohydrates - biosynthesis; Carbohydrates - chemistry; Cellulose - chemistry; Cellulose - metabolism; Enzymatic hydrolysis; Enzyme Activation; Food industries; Fundamental and applied biological sciences. Psychology; Glycoside Hydrolases - chemistry; Hydrolysis; Pectinase; Polygalacturonase - chemistry; Saccharum - microbiology; Trichoderma - chemistry; Trichoderma - classification; Trichoderma - metabolism; Trichoderma harzianum; Use and upgrading of agricultural and food by-products. Biotechnology; α-l-arabinofuranosidase
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.12.105

► The Amazon rainforest Trichoderma harzianum is a potential candidate for saccharification of plant biomass. ► Important roles for pectinase and α-l-arabinofuranosidase in the enzymatic synergies. ► Sugarcane bagasse hydrolysis is more effective and competitive for second generation ethanol. Supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. This study investigated which enzymes were complementary to the enzyme set of Trichoderma harzianum in the degradation of sugarcane bagasse. Specific activities of T. harzianum extract on different substrates were compared with the extracts of Penicillium echinulatum and Trichoderma reesei, and two commercial cellulase preparations. Complementary analysis of the secretome of T. harzianum was also used to identify which enzymes were produced during growth on pretreated sugarcane bagasse. These analyses enabled the selection of the enzymes pectinase and α-l-arabinofuranosidase (AF) to be further investigated as supplements to the T. harzianum extract. The effect of enzyme supplementation on the efficiency of sugarcane bagasse saccharification was evaluated using response surface methodology. The supplementation of T. harzianum enzymatic extract with pectinase and AF increased the efficiency of hydrolysis by up to 116%.