Thermogravimetry and Py–GC/MS techniques as fast qualitative methods for comparing the biochemical composition of Nannochloropsis oculata samples obtained under different culture conditions

► TGA and Py–GC/MS for biochemical composition analysis. ► Biochemical composition by usual methods highly correlated with TGA and Py–GC/MS. ► TGA and Py–GC/MS fast methods to compare the composition of different samples. Microalgae have many applications, such as biodiesel production or food supple...

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Bibliographic Details
Published in:Bioresource technology 2013-03, Vol.131, p.86-93
Main Authors: Valdés, F., Catalá, L., Hernández, M.R., García-Quesada, J.C., Marcilla, A.
Format: Article
Language:English
Subjects:
Biochemical composition
biodiesel
Biological and medical sciences
Biopolymers - analysis
Biopolymers - chemistry
carbohydrate content
carbohydrates
detectors
dietary supplements
Fundamental and applied biological sciences. Psychology
gas chromatography
Gas Chromatography-Mass Spectrometry - methods
Heating - methods
lipids
microalgae
proteins
Pyrolysis
qualitative analysis
Species Specificity
spectrometers
Stramenopiles - classification
Stramenopiles - metabolism
Thermogravimetric analysis
thermogravimetry
Thermogravimetry - methods
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Summary:► TGA and Py–GC/MS for biochemical composition analysis. ► Biochemical composition by usual methods highly correlated with TGA and Py–GC/MS. ► TGA and Py–GC/MS fast methods to compare the composition of different samples. Microalgae have many applications, such as biodiesel production or food supplement. Depending on the application, the optimization of certain fractions of the biochemical composition (proteins, carbohydrates and lipids) is required. Therefore, samples obtained in different culture conditions must be analyzed in order to compare the content of such fractions. Nevertheless, traditional methods necessitate lengthy analytical procedures with prolonged sample turn-around times. Results of the biochemical composition of Nannochloropsis oculata samples with different protein, carbohydrate and lipid contents obtained by conventional analytical methods have been compared to those obtained by thermogravimetry (TGA) and a Pyroprobe device connected to a gas chromatograph with mass spectrometer detector (Py–GC/MS), showing a clear correlation. These results suggest a potential applicability of these techniques as fast and easy methods to qualitatively compare the biochemical composition of microalgal samples.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.12.133